日用化学工业Issue(2)：106-110,5.DOI:10.13218/j.cnki.csdc.2016.02.010
结合组织学评分的牛角膜浑浊和渗透性方法研究
Research on bovine corneal opacity and permeability test integrating with histopathological evaluation
摘要
Abstract
In order to refine the alternative method for animal eye irritation test———the bovine corneal opacity and permeability (BCOP)test,and to enhance its application scope and predictability,the fresh bovine corneas were chosen and directly exposed to liquid chemical material to be tested for 10 min,post - incubated for 2 h and further treated with fluorescein sodium for 90 min. Then opacity of the treated corneas was measured with opacitometer;the permeability was determined with the multiskan spectrum microplate spectrophotometer and the histopathological changes of the cornea were observed and graded after routinely fixed and paraffin embedding. Finally,the predictive models based on the above three indices were established. The results showed that the corneal opacity and permeability,integrated with histopathological changes of epithelium, stroma and endothelium,the scope of irritation from mild level to moderate level can be accurately distinguished. In conclusion,the refined BCOP method integrated with histological observation could improve its predictability and suitable for study of eye irritation mechanism and cosmetic product evaluation.关键词
化妆品/眼刺激/替代方法/牛角膜浑浊和渗透性实验/组织学Key words
cosmetics/eye irritation/alternative method/BCOP/histopathology分类
化学工程引用本文复制引用
陈彧,喻欢,程树军,谈伟君,秦瑶..结合组织学评分的牛角膜浑浊和渗透性方法研究[J].日用化学工业,2016,(2):106-110,5.基金项目
广东省科技计划资助项目 （）
