山东医药Issue(1)：1-4,4.DOI:10.3969/j.issn.1002-266X.2016.01.001
丹皮酚对肝癌 HepG2／ADM 细胞株多药耐药性的逆转作用及其机制
Reversal effect of paeonol on multidrug resistance of liver cancer cell line HepG2/ADM
摘要
Abstract
Objective To investigate the reversal activity of paeonol on multidrug resistance of liver cancer cell line HepG2/adriamycin ( ADM) and its potential molecular mechanism.Methods The human HepG2 cell line was used to make the HepG2/ADM model which was induced by the increased concentrations of adriamycin (ADM).MTT assay was performed to detect the proliferation of HepG2/ADM and HepG2.The flow cytometer was used to measure the intracellular accumulation of cytotoxic drugs in HepG2/ADM and HepG2.Western blotting was carried out to determine the levels of P-glycoprotein.Results Paeonol with the concentrations from 0 to 50 μmol/L had no cytotoxicity to the HepG2/ADM and HepG2.The IC50 values for inhibiting proliferation of HepG2/ADM and HepG2 were 1.48 μM and 124.14 μM, and the resistance index was 83.9.Paeonol (25 μmol/L, 50 μmol/L) could significantly enhance the cytotoxic effects of ADM and 5-fluorouracil on HepG2/ADM, and therefore reduced their IC50 values (P<0.01), while It had no effect on those of HepG2.Moreover, Paeonol increased the accumulation of ADM in HepG2/ADM ( all P<0.01) , but not in HepG2.The further study found that Paeonol obviously reduced the expression level of P-glycoprotein in HepG2/ADM (all P<0.01), while had no effect on that of HepG2.Conclusion Paeonol can reverse the multidrug resistance of HepG2/ADM signifi-cantly, which may be related with the inhibition of P-glycoprotein expression and the increased accumulation of cytotoxic drugs.关键词
肝肿瘤/HepG2/阿霉素细胞株/丹皮酚/多药耐药/P-糖蛋白Key words
liver neoplasms/HepG2/ADM cell line/paeonol/multidrug resistance/P-glycoprotein分类
医药卫生引用本文复制引用
孙爱华,陈劲,关恒明,李平..丹皮酚对肝癌 HepG2／ADM 细胞株多药耐药性的逆转作用及其机制[J].山东医药,2016,(1):1-4,4.基金项目
国家中医药管理局科研基金项目（2009030）。 （）
