山东医药Issue(1)：5-8,4.DOI:10.3969/j.issn.1002-266X.2016.01.002
JNK信号通路对肝癌小鼠PGE2及CD4＋CD25＋调节性T细胞表达的影响
Effects of JNK signaling pathway on expression of CD4 +CD25 +regulatory T cells and PGE2 in mice with liver cancer
摘要
Abstract
Objective To investigate the influence of c-Jun N-terminal kinases ( JNK) signaling pathway on PGE2 and CD4 +CD25 +T regulatory T cells expression of mice with liver cancer.Methods The liver cancer xenograft models of BALB/c mice were divided into the experimental group and control group.Mice in each group were treated with SP600125 polypeptides or normal saline respectively for 24 days.RT-PCR was used to detect the expression of JNK pathway signal, ELISA was used to detect the PGE2 levels, and flow cytometry and immunohistochemistry were used respectively to detect CD4 +CD25 +T regulatory T cells levels in the peripheral blood and liver cancer tissues.Results The expression of JNK signaling pathway in the experimental group was lower than that of the control group [(0.68 ±0.03) vs.(0.98 ±0.169), P<0.05].The level of PGE2 was lower in the experimental group as compared with that of the control group [(4.64 ± 0.36) ng/g vs.(6.64 ±1.24) ng/g, P<0.05].In the periphery blood, PGE2 level of the experimental group was lower than that of the control group [(76.57 ±10.16) pg/ml vs.(107.88 ±16.13) pg/ml, P<0.05].In the liver cancer tis-sues, the percentage of CD4 +CD25 +T regulatory T cells of the experimental group was decreased as compared with that of the control group [(14.37 ±3.35)% vs.(30.90 ±4.19)%, P<0.01].In the peripheral blood, the percentage of CD4 +CD25 +T regulatory T cells of the experimental group was lower than that of the control group [(3.20 ±0.12)%vs. (4.22 ±0.32)%, P<0.05].Conclusion When JNK signaling pathway was blocked, the expression level was down-regulated, and consequently the levels of CD4 +CD25 +T regulatory T cells decreased.关键词
肝肿瘤/c-Jun氨基末端激酶/前列腺素E2/调节性T细胞Key words
liver neoplasms/c-Jun N-terminal kinases/prostaglandins E2/regulatory T cells分类
医药卫生引用本文复制引用
蔡沈阳,潘武,冯玺,叶辉..JNK信号通路对肝癌小鼠PGE2及CD4＋CD25＋调节性T细胞表达的影响[J].山东医药,2016,(1):5-8,4.基金项目
四川省科技支撑计划项目（2013FZ0012）。 （）
