摘要
Abstract
Objective To observe the effects of phytic acid ketone on expression of HPV 16/18 E6/E7 mRNA and protein in human cervical cancer cells.Methods The Caski cells ( containing HPV16 ) and Hela cells ( containing HPV18) were cultivated in vitro and then divided into the experimental groups 1, 2, 3, 4 and the control group ( each group contained Caski cell line and Hela cell line).The experimental groups 1, 2, 3, 4 were respectively treated with dif-ferent concentrations of phytic acid ketone (58.6 mg/L, 117 mg/L, 586 mg/L, 5 860 mg/L) for 72 h.The levels of HPV16/18 E6/E7 mRNA were determined with real-time PCR.Western blotting was used to measure the expression levels of E6 and E7 proteins.Results E6/E7 mRNA and protein expression in the same type of cells of the experimental groups 1, 2, 3 and 4 was lower than that of the control group, and the E6/E7 mRNA and protein expression showed a decreasing tendency in the experimental groups 1, 2, 3 and 4 (all P<0.05).In each experimental group, E6/E7 mRNA expression in Caski cells was lower than that in Hela cells (all P<0.05).In the same type of cells of the experiential groups, the E6 mRNA expression was lower than that of E7 mRNA (all P<0.05).Conclusions Phytic acid ketone could down-regulate the expression of E6/E7 mRNA and protein in human cervical cancer lines, which was in a dose-dependent manner.The inhibitory effect of phytic acid ketone on HPV16 E6/E7 mRNA was stronger than that on HPV18 E6/E7 mRNA and the suppression of E6 probably played a leading role.关键词
植酸酮/人乳头瘤病毒16/人乳头瘤病毒18/E6蛋白/E7蛋白/宫颈癌/Caski细胞株/Hela细胞株Key words
phytic acid ketone/human papillomavirus 16/human papillomavirus 18/E6 protein/E7 protein/cervi-cal carcinoma/Caski cell line/Hela cell line分类
医药卫生
