陕西医学杂志Issue(2)：139-140,146,3.DOI:10.3969/j.issn.1000-7377.2016.02.004
肝窦内皮细胞凝集素抑制肝星形细胞增殖、胶原合成与分泌的实验研究
LSECtin inhibits the proliferation,synthesis and secretion of collagen in the HSCs
摘要
Abstract
Objective:To investigate the effects of LSECtin (1iver and lymph node sinusoidal endothelial cells lectin) on the proliferation ,synthesis and secretion of collagen in the HSC‐T6 hepatic stellate cells .Methods :HSC‐T6 cells from rats liver were cultured in vitro ,and assigned into control and LSECtin treatment group .MTT assay and H3‐TdR incorporation were performed to evaluate the proliferation of HSC‐T6 cells .[3 H]‐Pro incorpora‐tion and colorimetry methods were applied respectively to detect the synthesis and secretion of collagen in HSC‐T6 cells .Results:LSECtin inhibited the proliferation of HSC‐T6 cells in a dose‐dependent manner .The inhibition ratio of LSECtin at 10‐7 mol/L was 50% .In addition ,LSECtin inhibited the synthesis and secretion of collagen in the HSC‐T6 cells dose‐dependently .At the dose of 10‐7 mol/L ,LSECtin decreased the synthesis and secretion of collagen in the HSC‐T6 cells cells by 55% and 52% respectively .Conclution:LSECtin is an inhibitor of proliferation ,synthesis and secretion of collagen in the HSCs ,suggesting potential therapeutic values against liver fibrosis .关键词
肝星形细胞/细胞增殖/肝纤维化/肝窦内皮细胞凝集素Key words
Hepatic stellate cells/Cell proliferation/Liver fibrosis/LSECtin分类
医药卫生引用本文复制引用
孙安,范郁会,杨洁英,江玉刚,乔立敏,王继波,李元峰..肝窦内皮细胞凝集素抑制肝星形细胞增殖、胶原合成与分泌的实验研究[J].陕西医学杂志,2016,(2):139-140,146,3.基金项目
陕西省卫生和计划生育委员会科研计划项目 （）
