陕西医学杂志Issue(3)：259-262,4.DOI:10.3969/j.issn.1000-7377.2016.03.001
缓释硫化氢供体对小鼠脑缺血急性期保护作用实验研究
The Protective effects of a slow-releasing hydrogen sulfide donor to mice acute ischemia
摘要
Abstract
Objective :To investigate the effects of the slow-releasing hydrogen sulfide donor ADT-OH in mice cerebral ischemic reperfusion injury .Methods : The mice focal cerebral ischemia model was found through MCAO and the male ICR mice were randomly divided into Sham ,Veh ,and slow-releasing hydrogen sulfide donor ADT-OH group .After 24 hours'ischemic reperfusion ,the brain tissue was got out and the mice's brain infarct vol-umes were assessed with triphenyltetrazolium chloride (TTC) staining .The expression of inflammatory factors in tissue was detected by qPCR .Results :The TTC manifested that compared to Veh ,the ADT-OH could significantly reduce the cerebral infarct volume of the pallium ,internal capsule and cerebral hemisphere after the ischemic reper-fusion .The qPCR shown that compared to Sham ,the expression level of proinflammatory factors like iNOS ,IL-1β , TNF-α and IL-6 in the ischemic cortex in Veh was significantly increased ;while compared to Veh ,ADT-OH re-duced the expression of proinflammatory factors significantly .Conclusion :ADT-OH exerted protective effects to a-cute cerebral ischemic reperfusion injury .The underlying mechanism may be related to the inflammatory reaction which induced by the inhibition of cerebral ischemia .关键词
硫化氢/供体/脑缺血/病理生理学/再灌注损伤/炎症介导素类/小鼠Key words
Hydrogen sulfide/Donors/Brain ischemia/physiopathology/Reperfusion injury/In-lammation mediators/Mice分类
医药卫生引用本文复制引用
徐英秀,肖蕴祺,贾佳..缓释硫化氢供体对小鼠脑缺血急性期保护作用实验研究[J].陕西医学杂志,2016,(3):259-262,4.基金项目
国家自然科学基金资助项目 （）
