实用医学杂志2016，Vol.32Issue(1)：29-33,5.DOI:10.3969/j.issn.1006-5725.2016.01.010
一锅法合成近红外量子点用于胶质瘤荧光显像与光动力治疗
Near-infrared quantum dots through one-pot aqueous synthesization for bio-imaging and photodynamic therapy of gliomas
摘要
Abstract
Objective To synthesize a quantum dot (QD) to recognize glioma cells for imaging and photodynamic therapy. Methods By one-pot aqueous approach, near infrared-emitting CdTe was produced. After detection of its physicochemical characterizations, RGD was conjugated. Emission images were observed with confocal microscopy. To test its toxicity, CdTe-RGD with various concentrations was separately added into U251 and 3T3 cells for incubation in dark circulation. To test its photodynamic effect, U251 and 3T3 cells were then irradiated for 5 ~ 60 min using 632.8 nm laser. Results The QD (Φ = 3.75 nm, PL peak wavelength =700 nm, PLQY=20%) achieved was a spherical crystal with excellent monodispersity. Under confocal microscope , U251 cells were visualized but 3T3 cells not. In dark circulation, the survival rates of both U251 and 3T3 cells were above 85%. After laser irradiation, the survival rate of U251 cells decreased to (37 ± 1.6)%with the increasing of irradiation time and CdTe-RGD concentration. Conclusion With good physicochemical characterization and low toxicity, CdTe-RGD could be applied in biomedical imaging and photodynamic therapy of gliomas.关键词
胶质瘤/近红外/量子点/生物成像/光动力治疗Key words
Glioma/Near-infrared/Bioimaging/Photodynamic therapy/Quantum dots引用本文复制引用
刘明兴,仲骏,窦宁宁,夏磊,高国..一锅法合成近红外量子点用于胶质瘤荧光显像与光动力治疗[J].实用医学杂志,2016,32(1):29-33,5.基金项目
国家自然科学基金项目(编号:81471317) （编号:81471317）
上海市科学技术委员会引导类重点项目(编号:124119a0800) （编号:124119a0800）
