实用医学杂志2016，Vol.32Issue(1)：50-53,4.DOI:10.3969/j.issn.1006-5725.2016.01.016
双脱甲氧基姜黄素对黑色素瘤B16-F10细胞增殖、凋亡的影响
Effect of bisdemethoxycurcumin on the proliferation and apoptosis of melanoma B16-F10 cells
曾小飞 1马瑞东 1尚观胜 1付强 1李书平 1何小平1
作者信息
- 1. 610500 成都医学院第一附属医院胸心外科
- 折叠
摘要
Abstract
Objective To investigate the effect of bisdemethoxycurcumin on the proliferation and apoptosis of melanoma B16-F10 cells. Methods The B16-F10 cells were incubated with bisdemethoxycurcumin for 24 h, and MTT assay was used to detect the proliferation of B16-F10 cell. Flow cytometry was used to detect cell cycle and cell apoptosis. A C57BL/6 mouse melanoma model was established to investigate the effect of bisdemethoxycurcumin on the proliferation of melanoma. Expression of BCL-1 in B16-F10 cells and tissues was detected by western blotting assay. Results bisdemethoxycurcumin could significantly inhibit B16-F10 cell proliferation, induce B16-F10 cell apoptosis and block the cell cycle at S phase. The intravenous dosing of bisdemethoxycurcumin could inhibit the growth of melanoma. Bisdemethoxycurcumin could inhibit the expression of BCL-1. Conclusion Bisdemethoxycurcumin can inhibit the proliferation of B16-F10 cell, resulting from its role in promoting cell apoptosis.关键词
双脱甲氧基姜黄素/黑色素瘤B16-F10细胞/增殖/凋亡Key words
Bisdemethoxycurcumin/Melanoma B16-F10 cells/Fine breeding/Apoptosis引用本文复制引用
曾小飞,马瑞东,尚观胜,付强,李书平,何小平..双脱甲氧基姜黄素对黑色素瘤B16-F10细胞增殖、凋亡的影响[J].实用医学杂志,2016,32(1):50-53,4.
