现代妇产科进展2016，Vol.25Issue(2)：81-84,4.DOI:10.13283/j.cnki.xdfckjz.2016.02.001
Smac基因转染对卵巢癌SKOV3/DDP裸鼠移植瘤顺铂敏感性及微血管生成的影响
The effect of Smac on the sensitivity of nude mice xenografis of DDP-resistant ovarian cancer to cisplatin and angiogenesis
摘要
Abstract
Objective:To investigate the effect of Smac on the sensitivity of nude mice xenografis of DDP-resistant ovarian cancer to cisplatin and angiogenesis. Methods:Human cis-plantin-resistance ovarian cancer models transplanted subcutaneously in nude mice were estab-lished,and divided into 5 groups to do drug intervention:N. S. , DDP, empty plasmids and DDP,recobinant Smac plasmids as well as Smac plasmids and DDP were injeccted into the tumor-bearing mice with Lipofect to interfere with the growth of translanted tumor. The effect on the volume and inhibitory rate of the tumor were observed. The cell apoptosis in tumor tissues was detected by TUNEL. Immunohistochemistry was employed for calculating microvascular density( MVD) . Results:The tansplanted tumor volume in group Smac+DDP were significantly smaller than the other groups,the tumor inhabition rates of it was 72. 94%(P<0. 05). Each group,s apoptosis index detected by TUNEL was(19. 90±3. 18)%,(21. 82±4. 40)%,(22. 64 ±3. 81)%,(30. 23±4. 06)%,(48. 59±4. 47)%,MVD was(24. 8±5. 36),(21. 2±3. 11), (19. 8±3. 70),(14. 4±3. 85),(11. 6±2. 70). The apoptosis index of group Smac+DDP were highest and the MVD of it was lower than other four groups ( P<0 . 05 ) . Conclusions:Smac could enhance the sensitivity of ovarian carcinoma xenograff to cisplatin chemotherapy,and also can suppress tumor angiogenesis.关键词
卵巢癌/顺铂耐药/裸鼠/Smac/凋亡Key words
Ovarian cancer/Cisplatin resistance/Nude mice/Smac/Apoptosis分类
医药卫生引用本文复制引用
陈雨薇,张虹,杨超,强娟..Smac基因转染对卵巢癌SKOV3/DDP裸鼠移植瘤顺铂敏感性及微血管生成的影响[J].现代妇产科进展,2016,25(2):81-84,4.基金项目
天津卫生局科技基金重点项目(其他) （其他）
