现代妇产科进展2016，Vol.25Issue(2)：89-93,5.DOI:10.13283/j.cnki.xdfckjz.2016.02.003
HPV16变异体对HSIL锥切术后持续感染及复发的影响
The influence of HPV16 variants on the persistent infection and recurrence after HSIL conization
摘要
Abstract
Objective:To investigate the early warning role of E6 and L1 variants of HPV16 on the persistent infection and recurrence after cervical HSIL( High-grade squamous in-traepithelial lesion) conization. Methods:100 HSIL patients with HPV16 positive testing but negative incisal margin after LEEP( loop electrosurgical excision procedure) among the women who visited the Peopleˊs Hospital of Shanghai Pudong District during May 2011 to June 2012 were enrolled in this study. E6 and L1 genes of the cervical exfoliated cells of the patients were sequenced and the patients were followed up for two years. The above genes were sequenced once again to identify the HPV16 variants for the cases of persistent infection and recurrence. Results:HPVl6 variants were detected in all of the 100 patients,of which 81. 0% belonged to the Asian prototype ( As. P) ,14% belonged to the European variant,2% belonged to the Euro-pean prototype(EP),3% belonged to the Africa 1 variant (Af1). It was found thatHPV16 per-sistent infection and recurrence were more prone to arise for the European variants E6 T350G, A442C(P<0. 001). In the vast majority of samples (94. 0%),L1 gene variants H228D,T292A and 449insS appeared simultaneously,and the differences of L1 gene variants between the re-currence groups,persistent infection groups and viral clearance groups were not statistically sig-nificant(P>0. 05). Conclusions:(1)HPV16 variants were closely related to HSIL occurrence. ( 2 ) The HPV16 variants resulting in HSIL in Shanghai Pudong area belonged mostly to the Asi-an variants,(3)The European variants E6 T350G,A442C may be more inclined to be associat-ed with HPV16 persistent infection and recurrence after HSIL conization. (4) L1 gene variants donˊt seem to correlate with HPV16 persistent infection and recurrence after HSIL conization.关键词
人乳头瘤病毒/高度鳞状上皮内病变/E6,L1变异体Key words
Humall papillomavims/High-grade squamous intraepithelial lesion/E6/L1/Variant分类
医药卫生引用本文复制引用
张蕾,杨宾烈,原杰彦,曹惠敏,周爱枝..HPV16变异体对HSIL锥切术后持续感染及复发的影响[J].现代妇产科进展,2016,25(2):89-93,5.基金项目
上海市卫生局科研基金资助项目(No:20124485) （No:20124485）
上海市浦东新区领先人才科研基金资助项目(No:GWRI2010-04) （No:GWRI2010-04）
上海市浦东新区人民医院重点学科资助项目(No:PDRYZDXK2012-02) （No:PDRYZDXK2012-02）
