现代妇产科进展2016，Vol.25Issue(2)：112-115,119,5.DOI:10.13283/j.cnki.xdfckjz.2016.02.008
子宫内膜异位症裸鼠模型子宫内膜的雌、孕激素受体表达
Altered expression of progesterone receptor and estrogen receptor in eutopic endometri-um in a nude mouse model of endometriosis
摘要
Abstract
Objective:To observe gene and protein expression of progesterone receptor ( PRA,PRB) and estrogen receptor ( ERα, ERβ) in eutopic endometrium in a surgically-in-duced endometriosis nude mice model,and to explore a proper model for investigating the path-ogenesis of progesterone resistance in endometriosis. Methods:Adult nude mice were surgically induced endometriosis by intraperitoneal inoculation of eutopic endometrium collected from in-fertile women with endometriosis. After 1,2,and 3 months,the endometrium of nude mice were collected. Then quantitative real-time RT-PCR and Western blot were used to analyze the gene and protein expression of PR( PRA,PRB) and ER( ERα,ERβ) in endometrium of experimen-tal mice. Result:The expressions of PRB and ERα mRNA and protein statistically significant decreased at 3 month,while PR and ERβ expressions increased. The ratios of PRB/PRA and ERα/ERβ decreased, statistically significant at 3 month. Conclusions:Nude mouse model of endometriosis is the ideal model for the research of endometriosis, the endometrium of nude mouse exist progesterone resistance.关键词
子宫内膜异位症/裸鼠/动物模型/孕激素抵抗Key words
Endometriosis/Nude mice/Animal models/Progesterone resistance分类
医药卫生引用本文复制引用
冯雪,宋永,周敏,肖丽,朱慧莉,黄薇..子宫内膜异位症裸鼠模型子宫内膜的雌、孕激素受体表达[J].现代妇产科进展,2016,25(2):112-115,119,5.基金项目
国家自然科学基金(No:81370693) （No:81370693）
