中国组织工程研究Issue(2)：179-184,6.DOI:10.3969/j.issn.2095-4344.2016.02.005
骨桥蛋白及其受体在黄韧带骨化症黄韧带细胞中的表达及其意义
Expression and role of osteopontin and its receptors in ligmentum flavum cells derived from patients with ossification of ligamentum flavum
摘要
Abstract
BACKGROUND:The exact pathogenesis of ossification of ligamentum flavum has not been elucidated yet. And osteopontin may be an important factor involved in the ossification of ligamentum flavum. OBJECTIVE:To clarify the expression and significance of osteopontin and its receptors, CD44 and integrin-β3, in ligamentum flavum cels between normal controls and patients with ossification of ligamentum flavum.METHODS:Ligamentum flavum tissues were obtained from normal adult controls and adult patients with ossification of ligamentum flavum (n=8 per group) who underwent thoracic/lumbar posterior decompression surgery. Ligmentum flavum cels were separated, cultured and identifiedin vitro, and osteopontin, CD44, integrin-β3 were stained using immunocytochemistry method and observed under inverted phase contrast microscope. And the mRNA expressions of osteopontin, CD44, integrin-β3 were measured by RT-PCR. RESULTS AND CONCLUSION: Immunocytochemistry results showed that the stronger positive staining for osteopontin, CD44, integrin-β3 was observed in the ossification of ligamentum flavum group than the control group (P < 0.01). The mRNA expressions of osteopontin, CD44 and integrin-β3 were also higher in the ossification of ligamentum flavum group than the control group (P < 0.05). These findings indicate that osteopontin and its receptors, CD44 and integrin-β3, in ligamentum flavum cels may play an important role in ossification of ligamentum flavum.关键词
组织构建/组织工程/肌肉肌腱韧带/骨桥蛋白/CD44/整合素β3/黄韧带细胞/黄韧带骨化症/原代培养/细胞鉴定/免疫荧光染色/PCR/国家自然科学基金分类
医药卫生引用本文复制引用
许政,周盛源,李学斌,刘晓东,陈雄生,王智清..骨桥蛋白及其受体在黄韧带骨化症黄韧带细胞中的表达及其意义[J].中国组织工程研究,2016,(2):179-184,6.基金项目
国家自然科学基金(81171753)@@@@the National Natural Science Foundation of China, No.81171753 （81171753）
