现代医药卫生Issue(4)：481-483,486,4.DOI:10.3969/j.issn.1009-5519.2016.04.001
可溶性CD40配体对K562细胞增殖及生存素mRNA和IL-8表达的影响
Effect of soluble CD40 ligand on proliferation of K562 cells and expression level of survivin mRNA
摘要
Abstract
Objective To investigate the effect of soluble CD40 ligand(sCD40L) on the proliferation of human leukemia K562 cells and expression levels of surviving mRNA and IL-8. Methods The MTT assay was used to examine the effect of differ-ent concentrations of sCD40L on the cellular proliferation ,RT-PCR was used to determine the expression level of survivin mRNA;ELISA was used to detect the IL-8 expression. Results Different concentrations of sCD40L(0.5,1.0,1.5,2.0,2.5μg/mL) all had the inhibiting effect on K562 cell proliferation,moreover could decrease the expression levels of survivin and IL-8;the cellular proliferation inhibiting rate of the sCD40L cell group was higher than that of the K562 cell group ,while the expression levels of surviving mRNA and IL-8 were lower than those in the K562 cell group,the difference was statistically significant(P<0.05), moreover which showed the dose and time dependent manner. Conclusion sCD40L can significantly inhibit the proliferation of K562 cells in vitro with the dose and time dependent manner,sCD40L inhibiting the K562 cell proliferation is correlated with the down-regulation of surviving mRNA and IL-8 expression.关键词
K562细胞/细胞增殖/survivin基因/白细胞介素8/可溶性CD40配体Key words
K562 cells/Cell proliferation/Survivin gene/Interleukin-8/Soluble CD40 ligand引用本文复制引用
封忠昕,吴燕梅,陈琦,肖建辉,冯进..可溶性CD40配体对K562细胞增殖及生存素mRNA和IL-8表达的影响[J].现代医药卫生,2016,(4):481-483,486,4.基金项目
贵州省社会发展攻关项目（黔科合SY字[2014]3025号）。 （）
