医药导报Issue(3)：249-252,4.DOI:10.3870/j.issn.1004-0781.2016.03.008
倒心盾翅藤对肾小管上皮细胞损伤的保护作用
Preventive Effects of Aspidopterys obcordata on Renal Tubular Epithelial Cells Injury
摘要
Abstract
Objective To explore the preventive effects of different extracts from Aspidopterys obcordata on renal tubu-lar epithelial cells injury induced by sodium oxalate in vitro, and initially identify the effective part for treating urolithiasis. Methods The injury model of HK-2 cells induced by sodium oxalate was established to screen the active parts of Aspidopterys obcordata by testing the protective effects of different polarity extracts on HK-2 injury cells through MTT method. Results Dif-ferent extracts from Aspidopterys obcordata improved the activity of HK-2 injury cells, which were elevated to 86.17% and 95.42%by 0. 5 mg•mL-1 and 1 mg•mL-1 aqueous extract, respectively. And the activity reached to 93. 59% and 84. 77% by 0.5 mg•mL-1 and 1 mg•mL-1 50% alcohol extracts, reached above 81.56% by 95% alcohol extracts,all of which showed sig-nificant difference compared with the model group. The HK-2 cells viability were elevated to 82. 53% and 91. 58% by 0.5 mg•mL-1 and 1 mg•mL-1 95% alcohol extracted ethyl acetate parts,and increased to 77.24% and 87.22% by 0.5 mg•mL-1and 1 mg•mL-1 of 95% alcohol isolated n-butanol extracts, approached to 95.46% and 81.36% by 0.5 mg•mL-1 and 1 mg•mL-1 water extracts, all of which showed significant difference compared with the model control group. Conclusion The aqueous extracts and alcohol extracts from Aspidopterys obcordata have obvious preventive effects on HK-2 cells injury, among which the ethyl acetate extracts, n-butanol extracts and water extracts present the remarkable effects, which are supposed to be the active parts for inhibiting calcium oxalate stone formation in vitro.关键词
倒心盾翅藤/草酸钠/HK-2细胞/损伤,上皮细胞,肾小管/提取,不同溶剂Key words
Aspidopterys obcordata Hemsl.var.obcordata/Sodium oxalate/HK-2 cells/Injury,epithelial cells,renal tubu-lar/Extract,different solvent分类
医药卫生引用本文复制引用
宋美芳,李宜航,李学兰,张忠廉,吕亚娜,李光..倒心盾翅藤对肾小管上皮细胞损伤的保护作用[J].医药导报,2016,(3):249-252,4.基金项目
中央级公益性科研院所基本科研业务专项( YZYN-14-04，YZYN-15-06) （ YZYN-14-04，YZYN-15-06）
