局解手术学杂志Issue(2)：79-82,83,5.DOI:10.11659/jjssx.11E015166
大鼠体外循环脏器损伤模型的建立
Establishment of rat model of organ injury caused by cardiopulmonary bypass
摘要
Abstract
Objective A easier and faster rat model of organ injury caused by cardiopulmonary bypass was developed to provide a stable and economical experimental platform for the research of CPB ( cardiopulmonary bypass ) .Methods Twenty male Sprague-Dawley rats weight between (288.8 ±26.1)g were randomly divided into sham group and CPB group .The right external jugular vein and bilateral femoral arteries were punctured in sham group ,while the rats in CPB group underwent 60 minutes cardiopulmoanry bypass with the perfusion flow no less than 120 mL· kg-1· min-1 .And the weight ,blood pressure ,blood gas and electrolytes were monitored and analyzed throughout the experi -ment.The serum,lung,liver and kidney were collected at 72 h after the operation for further detection .Results The rat model of cardiopul-monary bypass was established successfully with 90%survival rate .The changes of blood gas during intraoperative and postoperative were in accordance with clinical reality and the injury of lung ,liver and kidney were confirmed by serum results and pathological changes .Conclu-sion The model has the characteristics of lower weight ,less priming solution ,no need for endotracheal intubation and easier to assemble the bypass line ,furthermore the methods of puncture and CPB were exhibited through a large number of pictures .As a consequence ,the difficul-ties of establishing the rat model of CPB were reduced significantly .The changes of pathophysiology during intraoperative and postoperative were similar to clinical realities ,the survival rate and organ injury were satisfactory .So it can be used as an effective tool for the research of organ injury and the evaluation of therapeutic strategies associated with CPB .关键词
体外循环/脏器损伤/大鼠/模型Key words
cardiopulmonary bypass/organ injury/rat/model分类
医药卫生引用本文复制引用
李宗杰,刘凯,李德闽..大鼠体外循环脏器损伤模型的建立[J].局解手术学杂志,2016,(2):79-82,83,5.基金项目
南京军区重大专项基金 （）
