中国免疫学杂志Issue(1)：9-12,18,5.DOI:10.3969/j.issn.1000-484X.2016.01.002
类风湿关节炎外周血lncRNA差异表达研究
Study on expression of long non-coding RNA in rheumatoid arthritis
摘要
Abstract
Objective:To investigate the expression profile variation of long non-coding RNA( lncRNA) in the peripheral blood mononuclear cells of rheumatoid arthritis ( RA ) and healthy controls, and explore the role of lncRNA in the pathogenesis of RA.Methods:A total of 12 RA patients and 11 age-matched healthy controls from University Hospital of Hubei University for Nationalities were recruited.Using lncRNA microarray technology to detect differently expressed lncRNAs in 3 cases of RA PBMCs and 3 cases of healthy controls.GO and Pathway analysis was performed.The coding-non-coding gene co-expression networks of lncRNA and mRNA was constructed based on the correlation analysis,and then searched lncRNA in pathogenesis of RA through the cis-analysis and trans-analysis.Results:A total of 1 615 deregulated lncRNAs and 878 deregulated mRNAs were detected in RA patients.GO analysis of different expressed mRNA may involve in metal ion binding,protein kinase binding,nucleotide binding,regulation of transcription,et al.Pathway analysis of different expressed mRNA may involve in TNF signaling pathway,B cell receptor signaling pathway,pancreatic cancer,system lupus erythematosus endometrial cancer,et al.REL,SMAD3 and ETS1 may play an important role in the pathogenesis and development of RA through cis-analysis and trans-analysis.As the NONHSAG027875,FR378506 and NONHSAT031501 also had the similar function,and they may be related to the pathogenesis and development of RA.Conclusion:Differentially expressed lncRNAs may exert a partial role in RA,and may provide potential targets for future treatment of RA.关键词
长链非编码RNA( lncRNA)/类风湿性关节炎/PBMCsKey words
Long non-coding RNA/Rheumatoid arthritis/Peripheral blood mononuclear cells分类
医药卫生引用本文复制引用
夏燕,冯佳,陈安平,杨年安,向阳,苏林冲,袁林..类风湿关节炎外周血lncRNA差异表达研究[J].中国免疫学杂志,2016,(1):9-12,18,5.基金项目
本文受国家自然科学基金（81360672）、风湿性疾病发生与干预湖北省重点实验室开放项目（OIR13003A）和湖北省医学领军人才培养工程专项经费资助。 （）
