中国免疫学杂志Issue(1)：69-73,78,6.DOI:10.3969/j.issn.1000-484X.2016.01.015
转录因子BACH2在冠心病患者外周血单个核细胞中的表达及意义
Expression of transcription factor BACH2 and its significance on PBMC in patients with coronary artery disease
摘要
Abstract
Objective:To investigate the expression of transcription factor BACH2 on PBMC in patients with coronary artery disease( CAD) and discuss its significance and role.Methods:The circulating levels of BACH2 and TLR4 in PBMC were evaluated by RT-PCR and Western blot separately.Serum levels of signature cytokines of T-helper cells,cytokines and ox-LDL were evaluated by ELISA.The data among groups were analyzed by one-way ANOVA.Correlation between variables were subjected to linear regression analysis.Results:The expression level of BACH2 down-regulated and TLR4 up-regulated significantly in PBMCs of ACS patients had significantly higher concentrations of serum ox-LDL,cytokines of TH1 cells,TH2 cells and TH17 cells and low level of cytokine of Treg cells.Negative correlation exists significantly between BACH2 and TLR4 in patients with CAD and between BACH2 and IFN-γ,IL-4 and IL-17,and between BACH2 and ox-LDL in serum.However, positive correlation exists significantly between BACH2 and TGF-β1.Conclusion:Increased serum concentrations of ox-LDL probably down-regulated the expression of BACH2 in ACS patients by activating TLR4 on PBMC, resulting in promoting production of pro-inflammatory cytokines and suppressing production of anti-inflammatory cytokine and then might cause ACS.关键词
冠心病/转录因子BACH2/Toll样受体4/炎症因子Key words
Coronary artery disease/Transcription factor BACH2/TLR4/Inflammatory cytokines分类
医药卫生引用本文复制引用
单圣帅,王博远,凯山,林吉斌,林静,李大主..转录因子BACH2在冠心病患者外周血单个核细胞中的表达及意义[J].中国免疫学杂志,2016,(1):69-73,78,6.基金项目
本文为国家自然科学基金资助项目（No．81370406）。 （）
