中国免疫学杂志Issue(1)：83-89,7.DOI:10.3969/j.issn.1000-484X.2016.01.018
黑龙江地区人群 HLA－A、B、DRB1高分辨等位基因及单体型多态性研究
Polymorphism research of HLA-A, B, DRB1 high resolution alleles and haplotypes in population from Heilongjiang
摘要
Abstract
Objective:To research the distribution features of HLA-A,B and DRB1 high resolution alleles and haplotypes in Heilongjiang population.Methods:PCR-SBT methods was applied for HLA-A,B,DRB1 high resolution genotyping of 13 670 unrelated and healthy donors in region of Heilongjiang,and haplotype frequencies were calculated by counting and maximum likelihood method. Results:A total of 286 HLA alleles were observed and the most frequent alleles(>0.1)were A*02∶01,A*24∶02,A*11∶01,DR*07∶01,DR*09∶01 and DR*15∶01.Among 1 087 kinds of HLA-A-B haplotype ,there were 22 kinds frequency higher than 0.01,and 267 kinds with statistically significant and positive linkage disequilibrium(ALD>0,HF≥1.09×10-4,χ2>3.84).Moreover,among 1 329 kinds of HLA-B-DRB1 haplotype,there were 19 haplotypes frequency higher than 0.01,and 357 kinds with statistically significant and positive linkage disequilibrium.1 348 kinds of A-B-DR Haplotype were informative with frequency≥1.66 ×10-4 in 4 428 haplotypes, and a total of 17 kinds of A-B-DR haplotype frequency higher than 0.005.Conclusion: Get the distribution features of HLA high resolution allele and haplotype in Heilongjiang population, and associated genetic parameters, Distribution of alleles and haplotypes close to northern Han population,but have their own distribution.关键词
HLA-A/B/DRB1/高分辨基因分型/基因/单体型频率Key words
HLA-A/B/DRB1/High resolution genotyping/Gene/Haplotype frequency分类
医药卫生引用本文复制引用
李鑫,丁镌,王鑫,侯玲,刘颖,刘杰..黑龙江地区人群 HLA－A、B、DRB1高分辨等位基因及单体型多态性研究[J].中国免疫学杂志,2016,(1):83-89,7.基金项目
本文为黑龙江省卫生厅科研课题（No．2012－082）。 （）
