中国药科大学学报Issue(1)：84-89,6.DOI:10.11665/j.issn.1000-5048.20160112
过氧化物酶6对紫外线致大鼠角膜损伤的治疗作用及机制
Effects and mechanism of peroxiredoxin-6 on uItravioIet induced corneaI injury in rats
摘要
Abstract
To investigate the therapeutic effect of peroxiredoxin-6(PRDX6)on ultraviolet-induced corneal injury in rats and explore the mechanism.The rat model of corneal injury was established by exposing to ultravio-let.Male wister rats were randomly divided into control groups,dexamethasone (DXM)groups and PRDX6 groups,the rats were administered four times a day and for 12 days.The corneal opacity was observed with a slit-lamp microscope.Histopathologic changes were observed with light microscopy.The content of corneal malonalde-hyde(MDA)was determined by thiobarbituric acid test and the total antioxidative capacity(TAOC)was detected by chemical colorimetric test.P38 MAPK signal pathway was detected with the method of Western blot and the gene expression of cytokines were measured by RT-PCR method.Compared with the control group,PRDX6 treat-ment significantly reduced corneal opacity,improved corneal pathology injury,decreased the MDA content and in-creased the TAOC.In the PRDX6 group the level of phosphorylated p38 protein was significantly lower than that in the control group.The gene expression of cytokine were different between control and PRDX6 groups(P <0.05).PRDX6 showed therapeutic effect in the rat model of ultraviolet-induced corneal injury.This maybe be concerned with that it could alleviated the oxidative damage,suppressed p38 MAPK phosphorylation and regulate the gene expression of cytokine.关键词
送货/物酶 6/紫外线/角膜/p38 MAPK/抗氧化/损伤Key words
peroxiredoxin-6/ultraviolet/cornea/p38 MAPK/antioxidation/injury分类
医药卫生引用本文复制引用
吕佳惠,姜敏敏,石慧,李建远,杜振宁..过氧化物酶6对紫外线致大鼠角膜损伤的治疗作用及机制[J].中国药科大学学报,2016,(1):84-89,6.基金项目
国家自然科学基金资助项目（No．81300738）This study was supported by the National Natural Science Foundation of China(No．81300738) （No．81300738）
