中国药房2016，Vol.27Issue(3)：378-380,3.DOI:10.6039/j.issn.1001-0408.2016.03.31
HPLC法测定藤梨根中表儿茶素的含量
Content Determination of L-epicatechin in Actinidia arguta by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for the content determination of L-epicatechin in Actinidiae arguta. METHODS:HPLC was performed on the column of Kromasil C18 with mobile phase of acetonitrile-0.2%Acetic acid solution(15:85,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 210 nm,column temperature was 25 ℃,and the volume injection was 10 μl. RESULTS:The linear range of L-epicatechin was 10.47-167.52 μg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2%;average recovery was 98.07%-101.71%(RSD=1.39%,n=6). CONCLUSIONS:The method is simple, accurate and reliable,and suitable for the content determination of L-epicatechin in A. arguta.关键词
高效液相色谱法/藤梨根/表儿茶素/含量测定Key words
HPLC/Actinidia arguta/L-epicatechin/Content determination分类
医药卫生引用本文复制引用
赫军,马秉智,梁莹莹,王晓雪,刘晓..HPLC法测定藤梨根中表儿茶素的含量[J].中国药房,2016,27(3):378-380,3.基金项目
北京市自然科学基金资助项目(No.7154237) （No.7154237）
中日友好医院青年课题(No.2014-1-QN-2) （No.2014-1-QN-2）
