中国药房2016，Vol.27Issue(3)：381-383,3.DOI:10.6039/j.issn.1001-0408.2016.03.32
HPLC法同时测定维舒膏中醋酸曲安奈德和维A酸的含量
Simultaneous Determination of Triamcinolone Acetonide Acetate and Tretinoin in Dimensional Ointment by HPLC
肖丽丽 1丁志军 1许时贵 1郭宜城 1王亚敏 1罗美兰1
作者信息
- 1. 江西省皮肤病专科医院制剂室,南昌 330000
- 折叠
摘要
Abstract
OBJECTIVE:To establish a method for the contents determination of triamcinolone acetonide acetate and tretinoin in Dimensional ointment. METHODS:HPLC was performed on the column of Diamonsil C18 with mobile phase of methanol-0.1%Phosphoric acid solution(87:13,V/V),detection wavelength was 254 nm(0-5 min)and 350 nm(5-20 min),at a flow rate of 1.0 ml/min,column temperature was 25℃,and volume injection was 10 μl. RESULTS:The linear range was 0.5-5 μg/ml for triamcin-olone acetonide acetate(r=0.999 9)and 4.96-49.6 μg/ml for tretinoin(r=0.999 8);RSDs of precision,stability and reproducibility tests were lower than 1%;recoveries were 98.37%-100.03%(RSD=0.58%,n=6)and 98.21%-100.38%(RSD=0.78%,n=6). CON-CLUSIONS:The method is simple,accurate and fast,and suitable for the contents determination of triamcinolone acetonide ace-tate and tretinoin in Dimensional ointment.关键词
高效液相色谱法/维舒膏/醋酸曲安奈德/维A酸/含量测定Key words
HPLC/Dimensional ointment/Triamcinolone acetonide acetate/Tretinoin/Content determination分类
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肖丽丽,丁志军,许时贵,郭宜城,王亚敏,罗美兰..HPLC法同时测定维舒膏中醋酸曲安奈德和维A酸的含量[J].中国药房,2016,27(3):381-383,3.
