中国药房2016，Vol.27Issue(3)：386-389,4.DOI:10.6039/j.issn.1001-0408.2016.03.34
离子色谱法测定骨瓜提取物注射液中糖的含量
Content Determination of Carbohydrates in Osseous and Cucumis Extract Injection by Ion Chromatography
张伟 1张彤 1任连杰 1吕雯 1韩春霞 2周长明1
作者信息
- 1. 北京市药品检验所,北京 100035
- 2. 赛默飞世尔科技(中国)有限公司,北京 100007
- 折叠
摘要
Abstract
OBJECTIVE:To establish a method for the content determination of carbohydrates in Osseous and cucumis extract injection. METHODS:Ion chromatography was conducted on the column of Dionex CarboPac PA20 with mobile phase of water-1 mol/L Sodium acetate solution-250 mmol/L Sodium hydroxide solution(gradient elution)at flow rate of 0.45 ml/min,column tem-perature was 35 ℃,and injection volume was 10 μl,detector was electrochemical analysis detector. RESULTS:The linear ranges of galactose,glucose,mannose,sucrose and fructose were 0.5-10.0μg/ml(r>0.998 0);RSDs of precision,stability and reproduc-ibility tests were lower than 2%;recovery was 94.8%-99.1%(RSD=0.52%-0.89%,n=9). CONCLUSIONS:The method is sim-ple,fast and accurate,and can be used for the determination of carbohydrates in Osseous and cucumis extract injection.关键词
离子色谱法/骨瓜提取物注射液/糖Key words
Ion chromatography/Osseous and cucumis extraction injection/Carbohydrates分类
医药卫生引用本文复制引用
张伟,张彤,任连杰,吕雯,韩春霞,周长明..离子色谱法测定骨瓜提取物注射液中糖的含量[J].中国药房,2016,27(3):386-389,4.
