中国药房2016，Vol.27Issue(3)：399-401,3.DOI:10.6039/j.issn.1001-0408.2016.03.39
两头尖的HPLC指纹图谱研究
Study on the HPLC Fingerprint of Anemone raddeana
张彦飞 1李智萌 1赵利利 1贡济宇 1蔡广知1
作者信息
- 1. 长春中医药大学药学院,长春 130117
- 折叠
摘要
Abstract
OBJECTIVE:To establish the HPLC fingerprint for Anemone raddeana. METHODS:HPLC was performed on the column of Phemomenex Gemini C18 with mobile phase of 0.1%phosphoric acid-acetonitrile(gradient elution)at a flow rate of 1 ml/min,the detection wavelength was 206 nm,the column temperature was 30℃,and the injection volume was 20μl. With the refer-ence of raddeanin A,13 batches of A. raddeana were analyzed,chromatographic fingerprint similarity evaluation system software was conducted for similarity analysis,and SPSS 13.0 was conducted for cluster analysis. RESULTS:There were 11 common peaks in the 13 batches of A. raddeana with similarity of higher than 0.90. According to the verification,the fingerprint and control fin-gerprint shows good consistency. The drugs in Huadian,Jiaohe, Tiangang,Shulan,Tonghua and Fusong of Jilin and Shangzhi of Heilongjiang were regarded as category 1,and in Harbin,Yabuli town and Yimianpo of Heilongjiang,Qingyuan of Liaoning,Ji-nan of Shandong were category 2. CONCLUSIONS:The established fingerprint can provide reference for the identification and quality evaluation of A. raddeana.关键词
两头尖/高效液相色谱法/指纹图谱Key words
Anemone raddeana/HPLC/Fingerprint分类
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张彦飞,李智萌,赵利利,贡济宇,蔡广知..两头尖的HPLC指纹图谱研究[J].中国药房,2016,27(3):399-401,3.
