摘要
Abstract
OBJECTIVE:To establish a method for the simultaneous determination of rhein,emodin,chrysophanol and phy-scion in Niuhuang shangqing pill. METHODS:HPLC was performed on the column was Kromasil C18 with mobile phase of metha-nol-0.5% phosphoric acid(75:25,V/V),the column temperature was 30 ℃,the flow rate was 1.0 ml/min,the detection wave-length was 230 nm,and the injection volume was 10μl. RESULTS:The linear range was 1.50-150 mg/L for rhein,0.95-95 for em-odin,1.30-130 for chrysophanol and 1.15-115 mg/L for physcion(r≥0.995 0);RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 97.03%-100.40%(RSD=1.23%,n=9),98.37%-102.50%(RSD=1.15%,n=9),98.03%-101.10%(RSD=1.05%,n=9)and 97.83%-104.50%(RSD=2.16%,n=9). CONCLUSIONS:The method is simple,stable with good re-producibility,and can be used for the contents determination of rhein,emodin,chrysophanol and physcion in Niuhuang shangqing pill.关键词
牛黄上清丸/蒽醌类化合物/高效液相色谱法Key words
Niuhuang shangqing pill/Anthraquinone compounds/HPLC分类
医药卫生
