中国药房2016，Vol.27Issue(3)：412-414,3.DOI:10.6039/j.issn.1001-0408.2016.03.44
复方亚麻酸软胶囊中大豆卵磷脂和γ-亚麻酸含量测定的方法学研究
Study on Contents Determination of Phospholipid andγ-linolenic Acid in Compound Linolenic Acid Soft Cap-sule
隋国奇 1蔡广知 1李连坤 1贡济宇1
作者信息
- 1. 长春中医药大学药学院,长春 130117
- 折叠
摘要
Abstract
OBJECTIVE:To establish a method for the contents determination of phospholipid and γ-linolenic acid in Com-pound linolenic acid soft capsule. METHODS:HPLC was conducted to determine the content of soybean lecithin;the column was Agilent TC-C18 with mobile phase of methanol-water(84:16,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 219 nm,the column temperature was 30 ℃,and the injection volume was 10 μl.Gas chromatography was conducted to determine the content of γ-linolenic acid in the preparation;the column was DB WAX,injector temperature was 210 ℃ by temperature pro-grammed,carrier gas was helium at a flow rate of 1 ml/min by split injection(split ratio of 1:30),and the injection volume was 0.02μl. RESULTS:The linear range was 4.64-16.24μg/ml for phospholipid(r=0.999 6)and 0.093 44-0.327 04μg/ml forγ-linole-nic acid methy ester(r=0.999 6);RSDs of precision,stability and reproducibility tests were lower than 3%;recoveries were 97.44%-99.36%(RSD=0.93%,n=6) and 97.22%-99.07%(RSD=1.01%,n=6),respectively. CONCLUSIONS:The method is simple,stable with good separation,and can be use for the contents determination of phospholipid and γ-linolenic acid in Com-pound linolenic acid soft capsule.关键词
复方亚麻酸软胶囊/气相色谱法/高效液相色谱法/大豆卵磷脂/γ-亚麻酸Key words
Compound linolenic acid soft capsule/Gas chromatography/HPLC/Phospholipid/γ-linolenic acid分类
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隋国奇,蔡广知,李连坤,贡济宇..复方亚麻酸软胶囊中大豆卵磷脂和γ-亚麻酸含量测定的方法学研究[J].中国药房,2016,27(3):412-414,3.
