中国药房Issue(6)：805-807,3.DOI:10.6039/j.issn.1001-0408.2016.06.27
HPLC法测定补骨脂-青龙衣酊中补骨脂素的含量
Content Determination of Psoralen in Psoralea-pericarpium Juglates Liniment by HPLC
摘要
Abstract
OBJECTIVE:To establish a method for the content determination of psoralen in Psoralea-pericarpium juglates lini-ment. METHODS:HPLC was performed on the column of Shim-pack VP-ODS C18 with mobile phase of methanol-water(60∶40, V/V)at a flow rate of 0.8 ml/min,detection wavelength was 246 nm,column temperature was 25 ℃,and injection volume was 20μl. RESULTS:The linear range of psoralen was 2.49-39.90 μg/ml;RSDs of precision,stability and reproducibility tests were lower than 2.00%;recovery was 97.23%-100.58%(RSD=1.17%,n=6). CONCLUSIONS:The method is fast,simple,accurate and re-liable,and can be used for the content determination of psoralen in Psoralea-pericarpium juglates liniment.关键词
高效液相色谱法/补骨脂/青龙衣/补骨脂素Key words
HPLC/Psoralea/Pericarpium juglates/Psoralen分类
医药卫生引用本文复制引用
唐菱,赵瑞亭..HPLC法测定补骨脂-青龙衣酊中补骨脂素的含量[J].中国药房,2016,(6):805-807,3.基金项目
重庆市卫生局科研资助课题 （）
