中国药理学通报Issue(2)：211-215,216,6.DOI:10.3969/j.issn.1001-1978.2016.02.013
沉默MALAT1基因对蜂毒素诱导HepG2细胞增殖和凋亡的影响
Effects of silencing MALAT1 on proliferation and apoptosis in HepG2 cells induced by Melittin
摘要
Abstract
Aim To investigate the effects of silencing MALAT1 gene on cell proliferation inhibition and apop-tosis induced by Melittin in human hepatocellular car-cinoma HepG2 cells. Methods The inhibitory rate of cell proliferation treated with Melittin in HepG2 cells was examined by MTT assay. Apoptotic rate was detec-ted by flow cytometry. The MALAT1 expression level in HepG2 cells was measured by qPCR. Specific siR-NAs were utilized to silence MALAT1 expression. The rates of cell proliferation inhibition and apoptosis in HepG2 cells treated with siRNA and Melittin were compared with those of Melittin alone. Results Melit-tin significantly suppressed the growth of HepG2 and induced cell apoptosis in a dose-dependent manner. Compared with normal liver cell lines, MALAT1 was highly expressed in HepG2 cells ( P<0. 05 ) . The ex-pression of MALAT1 in HepG2 cells was inhibited by Melittin, and the inhibitory rate increased with the in-crease of concentration. The rates of cell proliferation inhibition and apoptosis in HepG2 cells treated with siRNA and Melittin were significantly higher than those treated merely with Melittin. Conclusion Melittin can reduce the expression of MALAT1 and silencing MALAT1 can effectively promote proliferation inhibi-tion and apoptosis in HepG2 cells induced by Melittin.关键词
蜂毒素/长链非编码RNA/MALAT1/RNA干扰/细胞凋亡/HepG2细胞Key words
Melittin/long noncoding RNA/MALAT1/RNA silence/apoptosis/HepG2 cells分类
医药卫生引用本文复制引用
赵斌,吴毓婷,黄成,吕雄文,李俊..沉默MALAT1基因对蜂毒素诱导HepG2细胞增殖和凋亡的影响[J].中国药理学通报,2016,(2):211-215,216,6.基金项目
国家自然科学基金资助项目(No 81473268,81273526) （No 81473268,81273526）
高校博士点基金资助项目( No 20123420120001) （ No 20123420120001）
安徽省自然科学基金资助项目(No 1408085MKL31,1308085MH145) （No 1408085MKL31,1308085MH145）
