中国药理学通报Issue(2)：234-238,5.DOI:10.3969/j.issn.1001-1978.2016.02.017
Pyrin重组蛋白对肺纤维化大鼠VEGF/VEGFR2/MMP-9信号通路的影响
Effects of pyrin recombinant protein on VEGF/VEGFR2/MMP-9 signaling pathway in pulmonary fibrosis of rats
摘要
Abstract
Aim To study the effects of pyrin recombi-nant protein ( PRP ) on VEGF/VEGFR2/ MMP-9 sig-naling pathway in bleomycin-induced pulmonary fibro-sis of rats. Methods Sixty male Wistar rats were ran-domly divided into groups of control ( n=10 ) , model ( n=20 ) , PRP ( n=20 ) , and SU5416 ( n=10 ) . All the rats, except for those in control group, were estab-lished as the model of interstitial pulmonary fibrosis by perfusion of bleomycin (5 mg·kg-1 ) through tracheal intubation. From the second day after modeling, all rats were intragastrically administered with drugs or sa-line, according to different groups designed. The rats were sacrificed on the 14th and 28th day, and lung samples were taken out. The pathological changes of interstitial pulmonary fibrosis were observed by HE staining and Masson staining to evaluate the degree of alveolitis and pulmonary fibrosis. Expressions of VEGF, VEGFR2, MMP-9 protein and mRNA were de-tected by immunohistochemistry and RT-PCR. Results On the 14th and 28th day, the alveolitis, pulmonary fibrosis, expression of VEGF, VEGFR2, MMP-9 and mRNA increased significantly in the model group com-pared with in the control group ( P <0. 05 ) , and de-creased significantly in PRP group than those in the model group ( P <0. 05 ) . Conclusion PRP plays a role of anti-pulmonary fibrosis via the down-regulation of VEGF/VEGFR2/MMP-9 signaling pathway.关键词
Pyrin重组蛋白/肺纤维化/血管内皮生长因子/血管内皮生长因子受体2/基质金属蛋白酶-9/大鼠Key words
pyrin recombinant protein/pulmonary fi-brosis/VEGF/VEGFR2/MMP-9/rats分类
医药卫生引用本文复制引用
安钟健,金燕,延光海,齐鹏,郑明昱,李良昌,朴红梅..Pyrin重组蛋白对肺纤维化大鼠VEGF/VEGFR2/MMP-9信号通路的影响[J].中国药理学通报,2016,(2):234-238,5.基金项目
国家自然科学基金资助项目( No 81260016,30960140,81260665) （ No 81260016,30960140,81260665）
吉林省科技厅自然科学基金资助项目( No 201215235) （ No 201215235）
吉林省科技厅中青年科技创新领军人才及创新团队项目(No 20140519013JH) （No 20140519013JH）
