中国药理学通报Issue(2)：245-250,251,7.DOI:10.3969/j.issn.1001-1978.2016.02.019
党参、甘草糖提取物对小肠上皮细胞迁移多胺信号通路的影响
Effect of Codonopsis and Glycyrrhizae saccharide extracts on polyamine-dependent signaling pathway during cell migration in IEC-6
摘要
Abstract
Aims To observe the effect of saccharide extracts of Yiqijianpi herb Codonopsis and Glycyrrhizae on polyamine-dependent activation of K+ channels sig-nal pathway during cell migration and to investigate their mechanism of promoting restoration in gastrointes-tinal mucosal injuries. Method The study was based on IEC-6 cell migration model. While in a normal polyamine level or polyamine was inhibited by DFMO, the effect of Codonopsis saccharide extracts and Glycyr-rhizae saccharide extracts on polyamine-dependent acti-vation of K+ channels signal pathway during cell mi-gration was observed. (1) K+ channel protein Kv1. 1 was determined by Western blot. (2)Membrane poten-tial was measured by Flow Cytometer. (3) Laser scan-ning confocal microscope was used for measuring [ Ca2+] cyt. ( 4 ) The expression of RhoA, which is Ca2+ downstream protein, was determined by Western blot. Results During cell migration, Codonopsis and Glycyrrhizae saccharide extracts could: ( 1 ) improve the expression of Kv1 . 1 protein and ameliorate the de-crease of kv1. 1 protein expression by DFMO;(2) in-crease membrane hyperpolarization and reverse mem-brane depolarization resulted by DFMO; ( 3 ) improve intracellular [ Ca2+] cyt, while Codonopsis could re-verse the decrease of [ Ca2+] cyt caused by DFMO;(4) improve the expression of RhoA protein, reversing its decline caused by DFMO. Conclusion Codonop-sis and Glycyrrhizae saccharide extracts can promote cell migration in IEC-6 cell, which is correlated with their effect on polyamine-dependent activation of K+channels signal pathway.关键词
党参/甘草/糖提取物/小肠上皮细胞/迁移/作用机制Key words
Codonopsis/Glycyrrhizae/saccharide ex-tracts/intestinal epithelial cell/migration, mechanism of action分类
医药卫生引用本文复制引用
李茹柳,陶玉珠,曾丹,赵世清,林传权,陈蔚文..党参、甘草糖提取物对小肠上皮细胞迁移多胺信号通路的影响[J].中国药理学通报,2016,(2):245-250,251,7.基金项目
国家自然科学基金资助项目(No 30772753,81173254) （No 30772753,81173254）
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