中国药理学通报Issue(2)：251-257,7.DOI:10.3969/j.issn.1001-1978.2016.02.020
海兔素对大鼠原代肝细胞酒精性氧化损伤的保护作用
Effects of Aplysin on ethanol-induced oxidative damage in rat primary hepatocytes
摘要
Abstract
Aim To investigate the protective effects of Aplysin on ethanol-induced oxidative damage in rat pri-mary hepatocytes. Methods Rat primary hepatocytes were obtained via the portal vein collagenaseⅣin situ perfusion technique followed by a Percoll density gradi-ent centrifuge. MTT test was used to determine the op-timum dose of Aplysin and ethanol, and detect the cell vitality in primary hepatocytes. Supernatants of primary hepatocytes were harvested to measure AST and LDH level, and the SOD, GSH-PX activities and MDA con-tent in primary hepatocytes were observed. Flow cy-tometry was used to detect the cell apoptosis rate. DNA damage in primary hepatocytes was detected by single-cell gel electrophoresis assay. The level of mitochon-drial membrane potential in primary hepatocytes was tested by fluorogenic probe JC-1 . The CYP2 E1 activity in primary hepatocytes was detected by colorimetry. The proteins of CYP2 E1 were detected by Western blot. Results 300 mmol·L-1 dose of ethanol and 30 mg·L-1 dose of Aplysin were the optimal dosages and were used in the subsequent experiments. Hepatocyte vitality was significantly increased in Aplysin group compared to that in ethanol group, and Aplysin inhibi-ted the release of AST and LDH(P<0. 05). For Apl-ysin treatment group, the activities of hepatocyte SOD and GSH were significantly increased, and MDA was markedly lowered as compared with those in ethanol group( P <0. 05 ) . Aplysin could alleviate hepatocyte apoptosis significantly, and hepatocyte DNA damage rates of Ⅱ ~Ⅲ level and Ⅳ level were significantly lowered in Aplysin treatment group as compared with those in ethanol group, and Aplysin had evident im-provement in alcohol induced mitochondria damage of hepatocyte. Primary hepatocyte activities and protein expression of CYP2 E1 were markedly lowered in Aply-sin treatment group as compared with those in ethanol group(P<0. 05). Conclusion Aplysin has protective effects on liver oxidative damage induced by alcohol of primary cultured rat hepatocytes by blocking CYP2 E1 activation, relieving oxidative stress, and sharpening the oxidation resistance ability.关键词
海兔素/大鼠/原代肝细胞/酒精性损伤/抗氧化/凋亡/CYP2E1Key words
Aplysin/rat/primary hepatocytes/alco-holic damage/antioxidation/apoptosis/CYP2 E1分类
医药卫生引用本文复制引用
苏爱,朱红燕,徐宏伟,刘颖,梁惠..海兔素对大鼠原代肝细胞酒精性氧化损伤的保护作用[J].中国药理学通报,2016,(2):251-257,7.基金项目
国家自然科学基金资助项目(No 31171671,81573137) （No 31171671,81573137）
达能营养中心膳食营养研究与宣教基金( No DIC2014-03) （ No DIC2014-03）
青岛大学创新型教学实验室研究项目 （）
