中国药理学通报Issue(2)：274-278,5.DOI:10.3969/j.issn.1001-1978.2016.02.024
小鼠不同心肌肥厚模型标志性基因表达变化的比较
Comparison of marker gene expression changes in different mouse models of cardiac hypertrophy
摘要
Abstract
Aim To explore the differences in hyper-trophic marker genes such as atrial natriuretic peptide ( ANP) , brain natriuretic peptide ( BNP) and β-myo-sin heavy chain (β-MHC) genes in different models of cardiac hypertrophy. Methods Respectively using re-nal abdominal aortic coarctation ( AAC) , arteriovenous fistula ( AVF) and isoproterenol ( ISO) methods to es-tablish C57BL/6 mouse model of cardiac hypertrophy. After modeling, each mouse ’ s body weight ( BW ) , heart weight ( HW) and left ventricular weight ( LVW) were weighed, and the heart weight ( HW/BW) and left ventricular index ( LVW/BW ) were calculated;myocardium by HE staining, pathological morphologi-cal changes were observed; myocardium by immuno-histochemistry, ANP, BNP and β-MHC protein ex-pression was observed;myocardium by Real-time PCR detection, ANP, BNP and β-MHC mRNA expression was observed. Results Compared with control group, HW/BW and LVW/BW were increased in three mod-els. Through the light microscope, each mouse model showed varying degrees of cardiac hypertrophy. ANP, BNP and β-MHC were increased in the protein and mRNA expression. Compared with AAC group, AVF and ISO groups’ myocardial tissue ANP, BNP and β-MHC expression were decreased in the protein and mRNA expression. Conclusions Three cardiac hy-pertrophy models are successful. Cardiac tissue ANP, BNP and β-MHC expression in AAC model exceeds AVF and ISO model.关键词
小鼠/心肌肥厚/心房利尿钠肽/脑利尿钠肽/β-肌球蛋白重链/模型Key words
mice/cardiac hypertrophy/ANP/BNP/β-MHC/model分类
医药卫生引用本文复制引用
阚红卫,司文文,尹艳艳,何灿,程杰,汪春彦,张琼光,杨雁..小鼠不同心肌肥厚模型标志性基因表达变化的比较[J].中国药理学通报,2016,(2):274-278,5.基金项目
安徽省自然科学基金资助项目(No 1308085MH144) （No 1308085MH144）
创新训练项目(No AH201310366005) （No AH201310366005）
