中国药理学通报Issue(2)：288-292,5.DOI:10.3969/j.issn.1001-1978.2016.02.027
不同品系小鼠肥胖哮喘模型建立及比较
Establishment and comparison of obese asthma models in different strains of mice
摘要
Abstract
Aim To establish and compare asthma models among different strains of obese mice. Methods Different strains of SPF female mice, namely Kunming ( KM ) , C57BL/6J and BALB/c mice, were randomly divided into four groups ( control group, asthma group, obesity group and obese asthma group). The mice were fed a high-fat diet or a normal diet for 12 weeks, following which they were sensitized and challenged with ovalbu-min ( OVA) or phosphate-buffered saline ( PBS) . Body weight, fat weight, liver weight, Lee′s index, OVA-specific IgE concen-tration in bronchoalveolar lavage fluid ( BALF) , serum total cho-lesterol ( TC) and triglyceride ( TG) levels, and lung and adi-pose morphologies were evaluated. The specific airway resistance ( sRaw) was measured using double-chamber plethysmography. Results The mice on a high-fat diet showed a more rapid in-crease in body weight compared with those on a normal diet. Af-ter 12 weeks of feeding, body, fat, and liver weights and Lee′s index were higher for the obese mice than for the lean mice. The adipocyte cross-sectional area was significantly greater in the obese BALB/c and KM mice than in their lean counterparts;the C57BL/6J groups showed no significant differences. The BALB/c mice demonstrated more significant symptoms of acute asthma, local inflammation and airway hyper-responsiveness ( AHR ) . Conclusion Compared with C57BL/6J and KM mice, BALB/c mice fed a high-fat diet and sensitized and challenged with OVA provide the most suitable model for evaluating the relationship between obesity and asthma.关键词
肥胖/哮喘/动物模型/KM 小鼠/C57BL/6J 小鼠/BALB/c小鼠Key words
obesity/asthma/model/KM mice/C57BL/6J mice/BALB/c mice分类
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陈一平,姜晓红,封广义,孙起翔,罗明洁,李超乾..不同品系小鼠肥胖哮喘模型建立及比较[J].中国药理学通报,2016,(2):288-292,5.基金项目
国家自然科学基金资助项目(No 81360007) （No 81360007）
