中国医学创新Issue(3)：1-4,4.DOI:10.3969/j.issn.1674-4985.2016.03.001
不同浓度、作用时间白花蛇舌草注射液对MG-63细胞凋亡效果
Effect of Different Concentration and Action Time Spreading Hedyotis Herb Injection Induced Mg-63 Cells Apoptosis
摘要
Abstract
Objective:To explore the effect of spreading hedyotis herb injection of bone sarcoma MG-63 cells apoptosis induction.Method:With a certain concentration of ethanol extraction of spreading hedyotis herb active ingredients, the mixture concentration of 50, 100, 200, 400 μL/mL four different spreading hedyotis herb injection. And its injection into different concentrations of bone sarcoma MG-63 cells in vitro culture. Determined by MTT method was used to detect the different concentration of spreading hedyotis herb injection effect on bone sarcoma MG-63 cells, 12, 24 and 48 h after cell proliferation inhibition rate, and USES the quantitative RT-PCR detection of apoptosis related gene Bax mRNA expression. Cells and decodes the (FCM) spreading hedyotis herb injection of bone sarcoma with MG-63 cell apoptosis rate.Result:Determined by MTT method to detect the result shows that, with the spreading hedyotis herb injection concentration and action time, increasing bone sarcoma with MG-63 cell proliferation inhibition rate were significantly increased, when the concentration of spreading hedyotis herb injection up to 100 μL/mL, with the duration extension, Bax gene expression was significantly higher (P<0.05). Flow cytometry instrument detection showed that after spreading hedyotis herb injection treatment, bone sarcoma with MG-63 cells apoptosis rate with the increase of the concentration of spreading hedyotis herb injection and rising.Conclusion:Spreading hedyotis herb injection via increased Bax gene expression can induce bone sarcoma with MG-63 cell apoptosis, and with the increase of concentration of spreading hedyotis herb injection, bone sarcoma with MG-63 cells apoptosis rate also increased.关键词
白花蛇舌草注射液/MG-63细胞/细胞凋亡Key words
Spreading hedyotis herb injection/MG-63 cells/Cell apoptosis引用本文复制引用
谢克恭,唐毓金,黄煜朗,黄可,陆路,林佳杰,卢贤哲..不同浓度、作用时间白花蛇舌草注射液对MG-63细胞凋亡效果[J].中国医学创新,2016,(3):1-4,4.基金项目
广西医学科学实验中心开放基金课题 （）
