中国医学创新Issue(5)：26-29,4.DOI:10.3969/j.issn.1674-4985.2016.05.007
百里醌抑制体外骨肉瘤细胞转移的实验研究
An Experimental Study on Anti-metastasis Effect of Thymoquinone on the Osteosarcoma SaOS-2 Cell Line in Vitro
周齐1
作者信息
- 1. 湖南省岳阳市一人民医院 湖南 岳阳 414000
- 折叠
摘要
Abstract
Objective:To investigate the anti-metastasis effect and its mechanism of thymoquinone on the osteosarcoma SaOS-2 cell line in vitro.Method:In the study,after application of different concentrations of thymoquinone in the role of human osteosarcoma SaOS-2 cells,the effects of thymoquinone on the migration and invasion of osteosarcoma SaOS-2 cells were examined by using wound assay and matrigel counting.Western blotting was used to detect the protein expression of MMP-2 and MMP-9 in SaOS-2 cells.Result:Thymoquinone significantly suppressed the migration and invasion of osteosarcoma SaOS-2 cells in a concentration depended manner.The expression of MMP-2 and MMP-9 were down-regulated in osteosarcoma SaOS-2 cells after treatment of thymoquinone.Conclusion:Thymoquinone exerts anti-metastatic activity in SaOS-2 cells in vitro,which may be achieved by down-regulating the expression of MMP-2 and MMP-9.关键词
骨肉瘤/百里醌/转移Key words
Osteosarcoma/Thymoquinone/Metastasis引用本文复制引用
周齐..百里醌抑制体外骨肉瘤细胞转移的实验研究[J].中国医学创新,2016,(5):26-29,4.
