中国肿瘤外科杂志Issue(1)：36-39,4.DOI:10.3969/j.issn.1674-4136.2016.01.011
高分辨率熔解曲线分析技术检测血清游离DNA的EGFR基因突变
High resolution melting analysis for EGFR mutations in plasma free DNA
摘要
Abstract
Objective To detect EGFR mutations using FFPE tissues and plasma free DNA by HRM analy-sis and to evaluate its clinical application value. Methods Formalin-fixed paraffin-embedded tissues and plas-ma free DNA of 200 NSCLC patients were detected by HRM analysis, the results were analyzed and compared accordingly. Results For FFPE tissues, the total EGFR mutation rate was 43. 5% detected by HRM, and for plasma free DNA, the EGFR mutation rate was 25. 0%. The sensitivity of HRM assays in plasma free DNA samples was 57. 5%, the specificity of HRM assays in plasma free DNA samples was 100%. Conclusions HRM for EGFR mutations in plasma free DNA could be accepted as an option for patients who are unable to ob-tain tumor tissue specimens in the diagnostic or screening setting.关键词
高分辨率熔解曲线分析技术/EGFR基因突变/血清游离DNAKey words
High resolution melting analysis/EGFR mutation/Plasma free DNA引用本文复制引用
王卓,井昶雯,吴建中,马蓉..高分辨率熔解曲线分析技术检测血清游离DNA的EGFR基因突变[J].中国肿瘤外科杂志,2016,(1):36-39,4.基金项目
江苏省肿瘤医院院内基金(ZQ201205,ZQ201206,ZM201203) （ZQ201205,ZQ201206,ZM201203）
