激光共聚焦检测双酚A致精母细胞损伤后线粒体膜电位的变化OACSTPCD
The changes of mitochondrial membrane potential detected by confocal microscopy in spermatocyte induced by bisphenol A
目的：运用激光扫描共聚焦显微镜(LSCM)技术检测双酚A(BPA)致精母细胞损伤后线粒体膜电位的变化，探讨其作用机制。方法：将培养的GC-2小鼠精母细胞分为对照组、低剂量组和高剂量组，分别加入0、10μmol/L、100μmol/L的BPA，一同培养3 h后使用荧光探针JC-1对3个组样本分别进行标记，采用LSCM检测胞内线粒体内JC-1荧光强度的变化。利用LSCM专用软件分析荧光强度，通过荧光颜色的转变检测线粒体膜电位的变化，用红绿荧光的相对比…查看全部>>
Objective:To detect changes in mitochondrial membrane potential after sperm mother cell damage induced by bisphenol A by laser scanning confocal microscopy(LSCM) and underline its potential action mechanism.Methods: The cultured spermatogenic cells were divided into 3 groups, respectively. Then 0, 10μmol/L, 100μmol/L of Bisphenol A(BPA) were added into the culture, after 3 hours culture, fluorescence probe JC-1 was used to lable the three groups. The fluores…查看全部>>
王丽婷;孙玮
第三军医大学生物医学分析测试中心 重庆 400038第三军医大学生物医学分析测试中心 重庆 400038
医药卫生
激光扫描共聚焦显微镜双酚A精母细胞线粒体膜电位荧光成像荧光探针JC-1
Laser scanning confocal microscopyBisphenol ASperm mother cellMitochondria membrane potentialFluorescence imagingFluorescence probe JC-1
《中国医学装备》 2016 (2)
106-109,4
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