摘要
Abstract
Objective To investigate the parameter range of the normal value and morphological features of bone marrow cells between Beagle dog and normal adult, and provide a diagnostic basis for the research that the target organs of test samples function in the preclinical study of new medicines is the safety evaluation research experiment of the pre-clinical medicines of hematopoietic system.Methods30 experimental control Beagle dogs (half male and half female) and normal adults' bone marrow smear were Rui-Ji mixed staining fluid, and morphologically observed. Under the oil microscopy, there were a total 500 nucleated cells, such as differential counting granulocyte system, erythrocyte system, lymphocyte system, monocyte system, plasmocyte system and other cells, which was expressed with a percentage. Under a high power microscopy, the number of megakaryocytes and calculate their myeloid erythroid ratio were counted. Made a pair comparison with SPSS19.0 and compute t value and P value.ResultsName of bone marrow cells in normal marrow picture of Beagle dogs, the classification of the change of bone marrow hyperplasia, and the classification of cell morphology of each system and phase of bone marrow cells basically referenced the classification standards of the human's bone marrow cells. P value of granulocyte system, erythrocyte system, megakaryocyte system and so on six big systems cells of Beagle dogs and normal adults was less than 0.01 or less than 0.0005, so there were significant differences. Under normal circumstances, the cell body of the bone marrow cells of homologous Beagle dog in the same phase was slightly smaller than people's, dogs' granulocyte system had a visible core-annulus, and their nuclear chromatin was rougher than people's, and their quality of cells was less rich than peoples' granulocytes; The mature lymphocytes of Beagle dog was usually less than 10%, and the mature lymphocytes of normal adults was between 20% and 30%; The number of granular megakaryocytes in born marrow picture of Beagle dogs was obviously larger than that of people, and there were usually osteoclasts. The myeloid erythroid ratio of Beagle dog could be counted as 2 to 2.2 times and people's myeloid erythroid ratio was 2 to 4 times.Conclusion There are differences in terms of the hyperplasia degree of bone marrow nucleated cells, the number and morphological features of nucleated cells (granulocytes, erythrocytes, megakaryocytes), lymphocytes, non-hematopoietic cells of Beagle dogs and normal adults. Through the comparison of bone marrow picture of dogs and normal adults, it provides referential data for an in-depth research that the medicines change the hematopoietic system.关键词
Beagle犬/正常成人/粒红比值/骨髓像Key words
Beagle dog/Normal adult/Myeloid erythroid ratio/Born marrow group分类
医药卫生
