作物学报2016，Vol.42Issue(3)：320-329,10.DOI:10.3724/SP.J.1006.2016.00320
小麦-黑麦易位系T1BL1RS在小麦品种中的分布及其与小麦赤霉病抗性的关联
Distribution of Wheat-Rye Translocation Line T1BL1RS in Wheat and Its Association with Fusarium Head Blight Resistance
摘要
Abstract
The short arm of 1R chromosome (1RS) of rye carries resistant genes to stripe rust, leaf rust, stem rust, powdery mil-dew and aphids. To understand if 1RS also mediates resistance to wheat Fusarium head blight (FHB), we genotyped a panel of 192 wheat accessions from diverse geographic regions and a population of recombinant inbred lines (RIL) consisting of 184 lines developed from the cross of Ning 7840 and Chokwang by 1RS-specific molecular marker Xscm9 and evaluated FHB severities in three consecutive seasons in greenhouses using single floret inoculation method. The results demonstrated that 22 of 192 acces-sions carried a T1BL×1RS translocation, and the mean FHB severity (PSS) of varieties carrying T1BL×1RS translocation was sig-nificantly lower than that of lines without the translocation across the three experiments (P<0.05), indicating 1RS may have a positive effect on reducing FHB severity. 1RS-specific marker Xscm9 and Genome in situ hybridization (GISH) showed Ning 7840 carries T1BL×1RS translocation. In the population of RILs, irrespective of Fhb1 locus, the mean PSS of lines with T1BL×1RS translocation was significantly lower than that of those lines without T1BL×1RS. The effects of Fhb1 and 1RS on FHB resistance were additive and the interactions between them were not significant (P=0.48). The results of this study suggested that 1RS of rye most likely carries the genes resistant to FHB.关键词
小麦/黑麦/T1BL-1RS易位系/赤霉病Key words
Wheat/Rye/T1BL-1RS translocation/Fusarium head blight引用本文复制引用
李韬,郑飞,秦胜男,李磊,顾世梁..小麦-黑麦易位系T1BL1RS在小麦品种中的分布及其与小麦赤霉病抗性的关联[J].作物学报,2016,42(3):320-329,10.基金项目
本研究由国家转基因生物新品种培育科技重大专项(2012ZX08009003-004),国家自然科学基金项目(31171537)和江苏省高校优势学科建设工程项目(PAPD)资助。This study was supported by the National Major Project of Breeding for New Transgenic Organisms (2012ZX08009003-004), the National Natural Science Foundation of China (31171537), and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) （2012ZX08009003-004）
