作物学报2016，Vol.42Issue(3)：330-343,14.DOI:10.3724/SP.J.1006.2016.00330
江淮稻区杂交粳稻骨干亲本产量性状配合力的SSR标记位点鉴定
Identifying SSR Marker Locus Genotypes with Elite Combining Ability for Yield Traits in Backbone Parents of Japonica Hybrid Rice (Oryza sativa L.) in Jianghuai Area
摘要
Abstract
Identifying marker loci related to combining ability (CA) for yield trait in parents of japonica hybrid rice facilitates improving CA of parents and enhancing standard heterosis degree of japonica rice by using molecular marker-assisted selection techniques. F1 seeds of 90 combinations were made by hand-crossed nine CMS lines with ten restorer lines using North Carolina Design II. The F1 populations were planted in Nanjing and Xuyi environments, and six yield traits were investigated. CA of the 19 parental lines was analyzed for six yield traits respectively using the data of 90 F1’s. Combining the data of CA and SSR marker genotypes of the 19 parental lines, SSR marker loci related to CA for six yield traits were detected. Results showed that BT-18A and Wuqiang A were elite CMS lines, and C418 was elite restorer lines in both environments. Number of detected SSR marker loci related to CA for effective panicles per plant, spikelets per panicle, filled grains per panicle, seed setting rate, 1000-grain weight and daily yield per plant were 8, 13, 11, 6, 6, and 2, respectively in Nanjing, 12, 21, 8, 15, 10, and 7, respectively in Xuyi, and 4, 11, 5, 3, 5, and 1, respectively in both environments. Heterozygous genotype marker loci showing positive heterosis ac-counted for 74%(34/46) in Nanjing, and 53%(39/73) in Xuyi. Among the SSR marker loci detected in both environments, three were each co-associated with CA for three yield traits, and another three for two yield traits. The remaining 14 marker loci were each associated with CA for one yield trait. Through data-base searching, genes/QTLs for the corresponding traits were found nearly ten of the marker loci detected in both environments. Strategies of enhancing CA for yield traits of restorer lines in japon-ica rice using the marker loci identified in this study were discussed.关键词
杂交粳稻/骨干亲本/产量性状配合力/标记位点基因型Key words
Hybrid Japonica rice/Backbone parents/Combining ability for yield traits/Marker locus genotypes引用本文复制引用
谢辉,党小景,刘二宝,曾思远,洪德林..江淮稻区杂交粳稻骨干亲本产量性状配合力的SSR标记位点鉴定[J].作物学报,2016,42(3):330-343,14.基金项目
本研究由国家高技术研究发展计划项目(2010AA101301),高等学校博士学科点专项科研基金(20110097110038,20130097110001)和中央高校基本科研业务费专项资金项目(KYZ201202-9)资助。This study was supported by China National High-tech Research and Development Program (2010AA101301), the Doctoral Found of Educational Ministry (20110097110038,20130097110001) and a grant from Educational Ministry of China for Basic Scientific Research of National Universities (KYZ201202-9) （2010AA101301）
