作物学报2016，Vol.42Issue(3)：344-352,9.DOI:10.3724/SP.J.1006.2016.000344
甘蓝型油菜结角高度与荚层厚度的全基因组关联分析
Genome-Wide Association Analysis of Height of Podding and Thickness of Pod Canopy in Brassica napus
摘要
Abstract
Layer of pod canopy is an important photosynthetic and seed storage part in rapeseed, providing important contribution to yield. In this study, 412 representative Brassica napus varieties (or lines) were genotyped using the Brassica 60 K Illumina Infinium SNP array by genome-wide association analysis of the height of podding (HP) and thickness of pod canopy (TPC). A total of 16 significant SNPs were identified, including two and four SNPs associated with HP and TPC in Chongqing, each of them explained 5.61–5.69%and 5.94–6.31%of phenotypic variation, respectively. Five and one significant SNPs accounting for 12.66–13.97%and 22.43%of the phenotypic variation for HP and TPC in Yunnan, respectively, were also detected. Three and one significant SNPs associated with the difference of HP and TPC between two environments were detected, explaining 17.33–20.32%and 29.05%of phenotypic variation, respectively. The latter SNP marker was located in the same linkage disequi-librium (LD) interval with one of significant SNPs related to TPC in Chongqing. Functional annotation of genes within the LD intervals containing significant markers showed that several genes involved in regulation of cell organization and biogenesis, flo-ral meristem development, number of silique, and multicellular organismal development existed, such as NSN1, TPST, and SAC1, which might result in the variation of HP and TPC through affecting the growth and development of flower or silique in B. napus. These loci and genes could be regarded as important candidate regions and genes for HP and TPC of B. napus. The results lay the foundation for reveal-ing the genetic basis and molecular mechanism for podding traits, and improving the yield per unit area of B. napus.关键词
甘蓝型油菜/全基因组关联分析/结角高度/结角层厚度/产量Key words
Brassica napus/Genome-wide association analysis/Height of podding/Thickness of pod canopy/Yield引用本文复制引用
卢坤,王腾岳,徐新福,唐章林,曲存民,贺斌,梁颖,李加纳..甘蓝型油菜结角高度与荚层厚度的全基因组关联分析[J].作物学报,2016,42(3):344-352,9.基金项目
本研究由国家重点基础研究发展计划(973计划)项目(2015CB150201),国家自然科学基金项目(U1302266,31401412),引进国际先进农业科学技术计划(948计划)项目(2011-G23),国家科技支撑计划项目(2013BAD01B03-12)和高等学校学科创新引智计划(111计划)项目(B12006)资助。This study was supported by the National Basic Research Program of China (973 Program)(2015CB150201), the National Science Founda-tion of China (U1302266 and 31401412), the Program of Introducing International Super Agricultural Science and Technology (948 Program)(2011-G23), the Key Technologies Research and Development Program of China (2013BAD01B03-12) and the 111 Project (B12006) （973计划）
