作物学报2016，Vol.42Issue(3)：353-360,8.DOI:10.3724/SP.J.1006.2016.00353
籼稻资源WD15515中抗褐飞虱QTL的定位研究
Analysis of QTLs for Brown Planthopper Resistance in Indica Rice WD15515
摘要
Abstract
Brown planthopper (BPH) is one of the most destructive insect pests of rice. WD15515 is an indica germplasm highly resistant to BPH. An F2 population was developed from the cross between 9311 and WD15515. One hundred F2 plants were genotyped by using SSR markers and evaluated for BPH-resistance by measuring the honeydew weight secreted by BPH (HW), the body weight increment (WB) and the body weight increment ratio (WRB). A total of four QTLs for BPH resistance were iden-tified. The qBph2-1, based on HW (honeydew weight), was detected between RM71 and RM6911 on chromosome 2, with LOD score of 3.68 and explaining the 11.08%of phenotypic variation. The qBph2-2, based on both WB and WRB, was mapped be-tween RM6911 and RM521 on chromosome 2, with LOD score of 3.31 and 4.05 and explaining 7.81%and 9.38%of the pheno-typic variation, respectively. The qBph4, based on HW, WB and WRB, was detected between RM16996 and RM17075 on chro-mosome 4, with LOD score of 11.11, 13.81, and 15.41 and explaining 44.38%, 45.24%, and 52.40%of the phenotypic variation, respectively. The qBph9, based on HW, WB, and WRB, was detected on chromosome 9 between RM219 and RM6444, with LOD score of 2.59, 4.04, and 3.63. This locus explained 10.91%, 12.39%, and 10.01%of the phenotypic variation in this population, respectively. qBph4 is a major gene for BPH-resistance. This result provides the new resources for BPH-resistance breeding.关键词
水稻/抗性/褐飞虱/数量性状基因座/遗传图谱Key words
Rice/Resistance/Brown planthopper/QTL/Genetic map引用本文复制引用
邓钊,何光存,石少阶,王卉颖,上官欣欣,刘丙芳,荆胜利,杜波,陈荣智,祝莉莉..籼稻资源WD15515中抗褐飞虱QTL的定位研究[J].作物学报,2016,42(3):353-360,8.基金项目
本研究由国家自然科学基金项目(31230060)和国家重点基础研究发展计划(973计划)项目(2013CBA01403)资助。This study was supported by the National Natural Science Foundation of China (31230060) and the National Basic Key Research Program (973 Program)(2013CBA01403) （31230060）
