作物学报2016，Vol.42Issue(3)：361-367,7.DOI:10.3724/SP.J.1006.2016.00361
携带抗白粉病基因Pm21的小麦-簇毛麦小片段易位染色体在不同小麦背景中的传递率及遗传稳定性
Transmission and Genetic Stability of No-homoeologous Small Fragment Wheat-Haynaldia villosa Translocation Chromosomes with Pm21 in Various Cultivar Backgrounds of Common Wheat
摘要
Abstract
The powdery mildew resistance gene Pm21 comes from a diploid wheat related species, Haynaldia villosa. Two Pm21-carrying small fragment translocation lines, the terminal translocation line NAU418 and the small interstitial translocation line NAU419, have been developed. Both lines are highly resistant to powdery mildew and serve as new genetic resources for improvement of disease resistance. For understanding the transmission rate of the translocation chromosomes through male and female gametes and the genetic stabilities in different wheat genetic backgrounds, the two translocations were crossed to 12 com-mon wheat varieties from different wheat growing areas of China. The F1 hybrids were then backcrossed as reciprocally. Chro-mosome configurations of the obtained F1s were analyzed by fluorescence in situ hybridization (FISH) of the PMC at MI. It was found that the translocation chromosomes formed rod bivalents with their corresponding wheat chromosomes. Test crosses showed that the translocation chromosomes T1AS×1AL-6VS and T4BS×4BL-6VS-4BL could be transmitted to their offspring. The transmission frequency of T1AS×1AL-6VS was higher through female gametes an average of 33.20%, ranging from 0.08% to 50.98%than through male gametes an average of 23.75%, ranging from 0.14%to 45.07%. Similarly, the transmission frequency of T4BS×4BL-6VS-4BL was higher through female gametes an average of 42.90%, ranging from 29.17%to 52.38%than through male gametes an average of 21.45%, ranging from 7.69%to 47.06%. These results show that the translocated chromosomes could be transmitted through male and female gametes, while genetic background has influences on the transmission rate, especially through male gametes.关键词
小麦/簇毛麦/白粉病/小片段易位/配子传递率Key words
Wheat/Haynaldia villosa/Powdery mildew/Small fragment translocation/Gamete transmission frequency引用本文复制引用
王海燕,肖进,袁春霞,徐涛,于春艳,孙昊杰,陈佩度,王秀娥..携带抗白粉病基因Pm21的小麦-簇毛麦小片段易位染色体在不同小麦背景中的传递率及遗传稳定性[J].作物学报,2016,42(3):361-367,7.基金项目
本研究由国家高技术研究发展计划(863计划)项目(2011AA100101-03,2011AA10010201),国家自然科学基金项目(31201204),中央高校基本科研业务费专项资金(KYZ201403)和江苏省优势学科建设工程项目(PAPD)资助。This study was supported by the National High Technology Research Program (863 Program) of China (2011AA100101,2011AA10010201), the National Natural Science Foundation of China (31201204), the Fundamental Research Funds for the Central Universities (KYZ201403) and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) （863计划）
