作物学报2016，Vol.42Issue(3)：368-375,8.DOI:10.3724/SP.J.1006.2016.00368
陆地棉GhRACK1启动子的克隆与缺失分析
Cloning and Deletion Analysis of GhRACK1 Promoter from Gossypium hirsutum
摘要
Abstract
The absence of fiber specific promoter with future prospect is one of the main factors to restrict the development of genetic engineering in cotton fiber improvement. A 1987 bp length promoter sequence of Gossypium hirsutum GhRACK1 gene, which encodes receptor for activated C kinase 1 and precedantly expresses in fiber, was cloned by combination of inverse PCR and touchdown PCR method. Sequence analysis showed there were lots of promoter regulation elements such as cis acting factor and the tissue specific regulation elements. The full-length GhRACK1-P and truncations from –600 to –1 bp, –1036 to –1 bp,–1260 to–1 bp and–1620 to–1 bp were obtained by PCR method. Each of the truncations was fused with gus gene and inserted into plant expression vectors pCamBIA2300. All constructs were transformed into Nicotiana tabacum var. NC89 through Agro-bacterium-mediated transformation method. GUS histochemical assay showed that the full-length GhRACK1-P promoter was expressed in root and exhibited a tissue-specific expression manner. All of the truncations were expressed in root, leaf and pollen and exhibited a constitutive expression manner. Because there is the similar developmental mechanism between cotton fiber and tobacco young root or trichome, the results indicate that GhRACK1-P may be a fiber specific expression promoter.关键词
纤维优势表达/GhRACK1启动子/克隆/缺失分析Key words
Preferential expression in fiber/GhRACK1 promoter/Clone/Deletion analysis引用本文复制引用
杨江涛,庞伟民,王旭静,吕少溥,唐巧玲,王志兴..陆地棉GhRACK1启动子的克隆与缺失分析[J].作物学报,2016,42(3):368-375,8.基金项目
本研究由国家转基因生物新品种培育重大专项(2014ZX08010-005)资助。This study was supported by the National Major Project of Breeding for New Transgenic Organisms (2014ZX08010-005) （2014ZX08010-005）
