作物学报2016，Vol.42Issue(3)：399-406,8.DOI:10.3724/SP.J.1006.2016.00399
青稞脂质转运蛋白基因blt4.9的克隆及其对非生物胁迫的响应
Isolation of blt4.9 Gene Encoding LTP Protein in Hulless Barley and Its Re-sponse to Abiotic Stresses
摘要
Abstract
Higher plant lipid transfer protein (LTP) is a class of small molecular weight alkaline single protein that can transfer phospholipids between biomembrane and form the biomembrane in cells. The objective of this study was to understand the func-tion of LTP gene in hulless barley (Hordeum vulgare L. var. nudum Hook. f.). The cDNA sequence of the LTP gene, blt4.9 (Gen-Bank accession number KU170187), was cloned from hulless barley variety Kunlun 12. The full length of blt4.9 cDNA is 720 bp including 348 bp of open reading frame and encodes 115 amino acids. The encoding product is a stable protein with a molecular weight of 11.2 kD, theoretical pI of 9.04, and instability coefficient of 28.41. This protein is rich in Gly, Ala, Leu, and Val amino acids excluding Trp, Glu, and Phe and similar to proteins encoded by other LTP genes. Sequence alignment indicated high simila-rity (98.3%) of protein encoded by blt4.9 from hulless barley and barley (Hordeum vulgare L.). The Real-time PCR assay showed that blt4.9 was up-regulated by 20–30%PEG-6000, 4ºC and 50mmol L-1 ABA and the expression level was higher in the most tolerant variety Handizi than in the most susceptible variety Dama, indicating a possible relationship between stress tolerance and blt4.9 in hulless barley. These results provide basic information in the utilization of LTP genes to improve hulless barley tolerance to abiotic stresses.关键词
青稞/blt4.9基因/克隆/非生物胁迫/基因表达量Key words
Hulless barley/blt4.9/Isolation of gene/Abiotic stresses/Gene expression level引用本文复制引用
姚晓华,吴昆仑..青稞脂质转运蛋白基因blt4.9的克隆及其对非生物胁迫的响应[J].作物学报,2016,42(3):399-406,8.基金项目
本研究由国家自然科学基金项目(31160284)和国家现代农业产业技术体系建设专项(CARS-05)资助。This study was supported by the National Natural Science Foundation of China (31160284) and the Special Program of Modern Agro-industry Technology System (CARS-05) （31160284）
