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瓜蒌皮总皂苷提取工艺优化及抑制金黄色葡萄球菌活性的研究

李婉珍 朱龙宝 陶玉贵 钱森和

安徽工程大学学报Issue(1):5-9,5.
安徽工程大学学报Issue(1):5-9,5.

瓜蒌皮总皂苷提取工艺优化及抑制金黄色葡萄球菌活性的研究

Optimization of extraction technology and studying on inhibiting of staphylococcus aureus activity in total saponins from trichosanthes kirilowii maxim peel

李婉珍 1朱龙宝 1陶玉贵 1钱森和1

作者信息

  • 1. 安徽工程大学微生物发酵安徽省工程技术研究中心,安徽芜湖 241000
  • 折叠

摘要

Abstract

In order to optimize the technology for extracting process of total saponins from Trichosanthes kirilowii Maxim peel ,on the bases of the single factor experiments for methanol concentration ,solid‐liq‐uid ratio ,extracting time ,extracting temperature and Ultrasonic power ,the orthogonal experiment L9 (34 ) was designed to optimize the extracting conditions of total saponins .The results showed that the optimal technology for extracting conditions of total saponins were the concentration of methanol 50% , the ratio of solid‐liquid of 1∶20 ,the extracting time of 50 min ,the Ultrasonic power of 150 W .The se‐quence of extraction process factors is the concentration of methanol> the extracting> the ratio of solid-liquid= the Ultrasonic power .The yield of total saponins increased from 0 .739% to 0 .986% .Mean while the anti‐microbial activity was carried on the extract in vitro ,The results showed that the saponins has inhibiting effect on Staphylococcus aureus ,with the bacteriostatic ring reaching 15 3. mm .

关键词

瓜蒌皮/总皂苷/超声波辅助法/抑菌活性

Key words

trichosanthes kirilowii maxim peel/total saponins/ultrasonic-assisted method/antimicrobial activity

分类

农业科技

引用本文复制引用

李婉珍,朱龙宝,陶玉贵,钱森和..瓜蒌皮总皂苷提取工艺优化及抑制金黄色葡萄球菌活性的研究[J].安徽工程大学学报,2016,(1):5-9,5.

基金项目

安徽省自然科学研究基金资助项目 ()

安徽工程大学学报

2095-0977

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