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黑曲霉固态发酵产柚苷酶培养基的优化

钱伟 黄元杰 王先锋 高乾坤 孟娜 魏胜华

安徽工程大学学报Issue(1):16-20,5.
安徽工程大学学报Issue(1):16-20,5.

黑曲霉固态发酵产柚苷酶培养基的优化

Medium optimization of naringinase production by Aspergillus niger in solid state fermentation

钱伟 1黄元杰 1王先锋 1高乾坤 1孟娜 1魏胜华1

作者信息

  • 1. 安徽工程大学生物与化学工程学院,安徽芜湖 241000
  • 折叠

摘要

Abstract

Naringinase was produced by solid fermentation of Aspergillus niger and the fermentation me‐dium was optimized by single factor experiments and orthogonal test as follows:in 500 mL erlenmeyer flask ,total 5 .5 g of bran and pomelo peel powder was used ,the amount of pomelo peel powder was 2 0. g ,the ratio of solid and liquid was 1∶2 5. ,0 5. g of ammonium sulphate was used as the best nitrogen source ,and 0 0.3 g of magnesium sulphate was used as the best inorganic salt .Under the optimized con‐ditions ,the fermentation activity of naringinase reached maximum 1248 0. U/g at 144 h ,which increased by 230% compared with the initial level (377 6. U/g) .

关键词

黑曲霉/柚苷酶/固态发酵/培养基优化

Key words

Aspergillus niger/naringinase/solid-state fermentation/optimization of the fermentation medium

分类

生物科学

引用本文复制引用

钱伟,黄元杰,王先锋,高乾坤,孟娜,魏胜华..黑曲霉固态发酵产柚苷酶培养基的优化[J].安徽工程大学学报,2016,(1):16-20,5.

基金项目

国家级大学生创新创业训练计划基金资助项目 ()

安徽工程大学学报

2095-0977

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