动物营养学报Issue(2)：468-476,9.DOI:10.3969/j.issn.1006-267x.2016.02.020
谷氨酰胺对黄颡鱼幼鱼生长性能、肠道形态及非特异性免疫相关基因表达的影响
Effects of Glutamine on Growth Performance, Intestinal Morphology and Non-Specific Immune Related Gene Expression of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)
摘要
Abstract
The effects of glutamine ( Gln) on growth performance, intestinal morphology and non-specific im-mune related gene expression of juvenile yellow catfish ( Pelteobagrus fulvidraco) were evaluated in this study. Two hundred and forty juvenile yellow catfish with the initial body weight of (2.49±0.04) g were randomly divided into four groups with three replicates, and there were twenty fish in each replicate.Four experimental diets were formulated to contain 0 ( control) , 0.10%, 0.20%and 0.40%Gln, which were randomly fed one of four groups for 70 days.The results showed that the growth performance indices were not significantly af-fected by Gln supplementation ( P>0.05) .Diets supplemented with Gln could improve the intestinal villus de-velopment, and increase the villus height and folds depth, but decrease the muscle thickness.Compared with control group, the intestinal villus height and folds depth in 0.20%and 0.40%groups were significantly in-creased ( P<0.05) , and the muscle thickness was significantly decreased ( P<0.05) .For the Gln supplemental level at 0.10%to 0.20%, the mRNA relative expression level of metallothionein ( MT) in muscle had the most obvious change, and that in supplementation groups was significantly higher than that in control group ( P<0.05); the mRNA relative expression level of glutathione-S-transferase ( GST) in intestine got the most obvi-ous change, and that in 0.20%group was significantly higher than that in control group ( P<0.05);the mRNA relative expression levels of heppatic bactericidal protein ( Hepc) and heat shock cognate protein 70 ( Hsc70) both in liver got the most obvious changes, the mRNA relative expression level of Hepc in 0.10%group was o-ver 73 times than that of control group ( P<0.05) , and the mRNA relative expression level of Hsc70 in 0.20%group was over triple than that of control group ( P<0.05) .In summary, these results suggest that Gln can im-prove the non-specific immune and antioxidant ability of juvenile yellow catfish at certain supplemental level.关键词
黄颡鱼/谷氨酰胺/非特异性免疫/肠道形态Key words
yellow catfish ( Pelteobagrus fulvidraco)/glutamine/non-specific immune/intestine morphology分类
农业科技引用本文复制引用
叶沙舟,张杰,陈海敏,周歧存,朱芳周..谷氨酰胺对黄颡鱼幼鱼生长性能、肠道形态及非特异性免疫相关基因表达的影响[J].动物营养学报,2016,(2):468-476,9.基金项目
宁波市创新团队（2011B81007）；浙江省重大科技专项（2012C12907-6）；国家公益性行业（海洋）科研专项（201105023）；国家海洋公益性行业科研专项 （）
