动物营养学报Issue(2)：477-484,8.DOI:10.3969/j.issn.1006-267x.2016.02.021
高通量测序分析人工养殖成年林麝粪便古菌菌群多样性
Fecal Archaeal Diversity of Artificial Breeding Adult Forest Musk Deer Analyzed by High-Throughput Sequencing Technologies
摘要
Abstract
This experiment was conducted to invest the archaeal structure and composition in feces of artificial breeding adult forest musk deer ( FMD) using high-throughput sequencing technologies, and to compare the difference between male and female FMD.Twelve healthy FMD with the age of three years were divided into male group ( M) and female group ( F) according to their gender ( 6 deer in each group) .The fresh feces were collected and DNA was extracted from them.The archaeal universal primers were used to amplify V4 to V5 regions of archaeal 16S rRNA.The amplifications were high-throughput sequenced with the MiSeq 300PE sequencing platform.The QIIME and other softwares were used to analyze the data.The results showed as fol-lows: from phylum to genus levels, the differences of the archaeal relative abundance between group M and group F were not significant ( P>0.05 ) .Genetic distance within the samples in group F and group M was 0.16±0.03 and 0.27 ±0.06, respectively, and that between groups was 0.24 ±0.07; all samples shared high similarity.The archaea in the feces of FMD can be divided into three phyla, the dominant phylum was Eur-yarchaeota; on the genus level they can be divided into seven existing genera, the dominant genus was Metha-nobrevibacter, the next one was Thermogymnomonas.In conclusion, the gender has no significant effect on the archaeal structure and composition in the feces of FMD, and Methanobrevibacter is the dominant genus.关键词
林麝/古菌/产甲烷古菌/多样性Key words
forest musk deer/archaea/methanogen/diversity分类
农业科技引用本文复制引用
王立志,徐谊英,蔡永华..高通量测序分析人工养殖成年林麝粪便古菌菌群多样性[J].动物营养学报,2016,(2):477-484,8.基金项目
科技部国际合作项目“畜禽低碳养殖关键调控技术合作研究” （）
