动物营养学报Issue(2)：507-514,8.DOI:10.3969/j.issn.1006-267x.2016.02.024
饲料中添加枯草芽孢杆菌对团头鲂幼鱼生长性能、肝脏抗氧化指标、肠道菌群结构和抗病力的影响
Effects of Dietary Bacillus subtilis on Growth Performance, Liver Antioxidant Ability, Intestinal Microflora Structure and Disease Resistance of Juvenile Blunt Snout Bream (Megalobrama amblycephala)
摘要
Abstract
This experiment was conducted to investigate the effects of dietary Bacillus subtilis ( B.subtilis) on growth performance, liver antioxidant ability, intestinal microflora structure and disease resistance of juvenile blunt snout bream ( Megalobrama amblycephala) .Three hundred and sixty juvenile blunt snout bream with an average body weight of (1.81±0.01) g were randomly chosen and divided into 4 groups with 3 replicates per group and 30 fish per replicate.Four isonitrogenous and isolipidic diets were formulated with the B.subtilis supplemental level was 0 ( T0 group, as control group), 2 ×107 ( T1 group), 2 ×108 ( T2 group), 2 × 109 CFU/g ( T3 group) , respectively, and those diets were randomly fed one of four groups for 8 weeks.The results showed as follows: 1) the weight gain rate ( WGR) and specific growth rate ( SGR) of T1 and T2 groups were significantly higher than those of control group ( P<0.05) , and the feed conversation ratio ( FCR) of T1 group was significantly lower than that of control group ( P<0.05 ) .2 ) Compared with the control group, the activities of liver antioxidant enzymes ( superoxide dismutase, catalase and catalase) of T1 group were significantly increased ( P<0.05) , meanwhile, the liver malondialdehyde content in that group was sig-nificantly decreased ( P<0.05) .3) No significant differences were observed in moisture, crude protein, crude lipid and ash contents of whole body among all groups ( P>0.05) .4) The denaturing gradient gel electrophore-sis ( DGGE) image demonstrated that the intestinal microflora structure was changed when juvenile blunt snout bream fed diets supplemented with different levels of B.subtilis.5) Following challenged by Aeromonas hy-drophila on the 96 h, fish fed different experimental diets showed no significant differences in cumulative mor-tality, but the cumulative mortality in T1 and T2 groups was lower than that in control and T3 groups.It is con-cluded that adding 2×107 CFU/g B.subtilis to juvenile blunt snout bream diet can improve growth performance and antioxidant ability, and change the intestinal microflora structure, but cannot significantly affect the body composition and disease resistance.关键词
团头鲂/枯草芽孢杆菌/生长/抗氧化能力/肠道菌群结构Key words
blunt snout bream ( Megalobrama amblycephala)/Bacillus subtilis/growth/antioxidant ability/intestinal microflora structure分类
农业科技引用本文复制引用
孙盛明,苏艳莉,张武肖,戈贤平,朱健..饲料中添加枯草芽孢杆菌对团头鲂幼鱼生长性能、肝脏抗氧化指标、肠道菌群结构和抗病力的影响[J].动物营养学报,2016,(2):507-514,8.基金项目
中央级公益性科研院所基本科研业务费专项资金（2015C06XK01）；江苏省自然科学基金（ SBK2015020058）；国家大宗淡水鱼类产业技术体系华东养殖岗位（CARS-46-14）；十二五国家科技支撑计划长江下游池塘高效生态养殖技术集成与示范 （）
