动物营养学报Issue(2)：555-563,9.DOI:10.3969/j.issn.1006-267x.2016.02.030
早期断奶对仔猪空肠和回肠兴奋性氨基酸载体1表达的影响
Effects of Early Weaning on Jenunal and Ileal Excitary Amino Acid Aarrier 1 Expression of Piglets
摘要
Abstract
This study was conducted to examine the effects of early weaning on adaptive changes in glutamate transporter gene expression in comparison with the suckling counterpart in the pig during the ages of 10 to 20 days.A total of 40 ternary hybrid piglets, with a similar body weight randomly taken from 40 different sows at 10 days of age, were divided into 2 groups.The trial group were 20 suckling piglets randomly obtained from 20 different sow litters, were allowed to continue suckling with their sows as the suckling group for 10 days;the 20 piglets of the weaning group were taken from another 20 different sow litters, moved off-site and weaned on a weaning diet for 10 days according to standard swine industry early weaning practices.At the end of the trial, 12 piglets were randomly taken from each group to remove the jejunum and ileum, and gene ex-pression of excitatory amino acid carrier 1 ( EAAC1) and free amino acids content were determined.The results showed that weaning significantly decreased the abundance of the EAAC1 ( 57 and 73 ku) and glutamate trans-porter associate protein 3-18 ( GTRAP3-18) ( 50 ku) protein and mRNA expression in jejunum and ileum of piglets ( P<0.05) .Weaning significantly increased the contents of glutamate and total free amino acids ( P<0.05) , while the contents of glutamate and total free amino acids in ileum were significantly decreased ( P<0.05).In conclusion, early weaning decrease jejunal and ileal EAAC1 and GTRAP3-18 protein expression and this may, in part, is related to early weaned piglets suffered from lack of nutrition leading to intestinal gluta-mate uptake disorder.关键词
仔猪/断奶/谷氨酸转运载体/EAAC1/小肠Key words
piglet/weaning/glutamate transporter/EAAC1/small intestine分类
农业科技引用本文复制引用
崔一喆,王秋菊,苏景,李悦,周亚强,陈玲..早期断奶对仔猪空肠和回肠兴奋性氨基酸载体1表达的影响[J].动物营养学报,2016,(2):555-563,9.基金项目
黑龙江省自然科学基金项目（C201444，C2015040）；黑龙江八一农垦大学校内课题资助（XZR2014-05）；中国博士后科学基金项目 （）
